N,O-羧甲基壳聚糖的制备及在抗菌竹纤维膜中的应用  

Preparation of N,O-carboxymethyl Chitosan and Its Application to Antibacterial Bamboo Fiber Film

作　　者：宋舒苹[1] 郑雪[1] 佘颖[1] 张浩[1] 蒲俊文[1] 吕卫军 

机构地区：[1]北京林业大学材料科学与技术学院,北京100083 [2]中国纸浆造纸研究院,北京100020

出　　处：《西南林业大学学报（自然科学）》2013年第1期105-108,共4页Journal of Southwest Forestry University：Natural Sciences

基　　金：林业公益性行业科研专项(201204702-B2)资助;北京市自然科学基金项目(2112032)资助

摘　　要：在弱碱性条件下,将氯乙酸与壳聚糖以一定比例混合,80℃下反应,制备高取代度的N,O-羧甲基壳聚糖。用红外光谱对N,O-羧甲基壳聚糖进行结构表征;用电位滴定法测定N,O-羧甲基壳聚糖的取代度;通过新型纤维素溶剂NaOH、尿素、硫脲、水溶解体系,将竹纤维与N,O-羧甲基壳聚糖以不同比例混合溶解制成竹纤维膜,用纺织品抗菌性测定方法测定不同N,O-羧甲基壳聚糖添加量的竹纤维膜对大肠杆菌、金黄色葡萄球菌的抑菌率。结果表明:添加N,O-羧甲基壳聚糖的竹纤维膜对金黄色葡萄球菌和大肠杆菌均具有良好的抑制作用,随着添加量的增加,竹纤维膜的抗菌性增强。N, O - carboxymethyl chitosan （ N, O - CMC） with high substitution was prepared at 80~C by mix- ing chitosan and monochloroacetic acid with different proportions under alkaline condition. Infrared Spectrum （ FT- IR） was used to identify the structure of N, O - CMC, and Potentiometric Titration was used to measure the substi- tution degree of N, O - CMC. N, O - CMC. Bamboo viscose cellulose and N, O - CMC was mixed by different proportions in the solution system of NaOH-urea-thiourea-water to get bamboo fiber film. The antibacterial property of bamboo fiber film against Escherichia coli and Staphylococcus aureus was measured by textiles evaluation for anti- bacterial activity-Shake Flask Method （ GB/T 20944.3 - 2008）. The results showed that the N, O - CMC added bamboo fiber film had good antibacterial property against Escherichia coli and Staphylococcus aureus. The antibacte- rial property of the blend membrane was improved when the proportion of N, O - CMC was increased.

关 键 词：N O-羧甲基壳聚糖 竹纤维膜 抗菌性 

分 类 号：S785[农业科学—林学]