应用痰模型涂片法检测抗酸杆菌结果分析  

The result analysis on the application of sputum membrane model on detecting mycobacterium tuberculosis

作　　者：刘庆福[1] 刘予东[2] 严秀丽[2] 邵德成 

机构地区：[1]山东省泰安市结核病防治中心参比实验室,271000 [2]山东省泰安市泰山区疾病预防控制中心结核病防治科,271000 [3]山东省泰安市宁阳县结核病防治所,271000

出　　处：《检验医学与临床》2013年第4期385-387,共3页Laboratory Medicine and Clinic

基　　金：山东省泰安市科技发展计划(编号:20113060)

摘　　要：目的评价痰模型涂片法检测抗酸杆菌的应用价值。方法应用组、研究组分别收集初诊活动性肺结核患者的痰标本780例、1 089例,应用模型涂片法检测抗酸杆菌,两组检测结果采用χ2检验进行比较。结果应用组与研究组应用痰模型涂片法检测抗酸杆菌的阳性检出率分别为29.74%(232/780)、31.68%(345/1 089)(χ2=0.799;P>0.05),差异无统计学意义,5项质量指标合格率均较高,分别为痰细胞(94.10%～94.21%)、痰膜大小(98.72%～98.99%)、厚薄(95.64%～96.14%)、染色(93.72%～94.49%)、痰膜脱落(97.18%～97.43%),两组痰涂片5项质量指标差异均无统计学意义(P值均大于0.05),说明应用组与研究组检测结果差异无统计学意义。结论痰模型涂片法检测抗酸杆菌,实现了涂片标准化,提高了制片质量和阳性检出率,灵敏度高,重复性好,实用性强,简单易行,特别适合于基层实验室应用。Objective To evaluate the application value of the establishing sputum membrane model to detect mycobacterium tuberculosis.Methods 1 089 and 780 sputum samples respectively from the initially diagnosed active pulmonary tuberculosis patients were collected as the experimental group and study group.The samples were detected with mycobacterium tuberculosis by sputum membrane model.Chi-square test was used to analyze the data.Results The experimental group and study group were detected the mycobacterium tuberculosis with the sputum membrane model respectively,and the positive rates were 29.74%（232/780） and 31.68%（345/1 089）（χ2=0.799;P〈0.05）.The difference had no statistical significance.The qualified rates of the five quality indicators were significantly high,namely the cells in sputum smear（94.10%-94.21%）,sputum membrane size（98.72%-98.99%）,thickness（95.64%-96.14%）,dyeing（93.72%-94.49%）,sputum membrane shedding pass rate（97.18%-97.43%）.There were no statistically significant differences between the five quality indicators of the two groups（P〈0.05）,indicating that the test results of two groups had no significant difference.Conclusion The application of sputum membrane model to detect mycobacterium tuberculosis achieves smear standardization,as well as improves producer quality and the positive rate.This model is of high sensitivity,good reproducibility,and it is practical,simple and particularly suitable for the primary laboratory applications.

关 键 词：分枝杆菌 结核 痰 细菌学技术 

分 类 号：R446.5[医药卫生—诊断学]