角碱蓬液泡膜Na^+/H^+逆向转运蛋白基因克隆及表达载体的构建  

Clone of Tonoplast Na^+/H^+ Antiporter Gene and Construction of Its Expression Vectors in Suaeda corniculata

作　　者：鞠会艳[1] 项剑[1] 

机构地区：[1]吉林大学植物科学学院,吉林长春130062

出　　处：《安徽农业科学》2013年第3期967-969,共3页Journal of Anhui Agricultural Sciences

基　　金：吉林省科技厅科技引导项目

摘　　要：[目的]克隆角碱蓬液泡膜Na+/H+逆向转运蛋白基因(ScNHX1),并构建其表达载体。[方法]以用400 mmol/L NaCl处理的角碱蓬为材料,利用RT-PCR技术从中克隆ScNHX1,并通过酶切、连接方法将ScNHX1连接至pCAMBIA1302载体中。[结果]试验成功克隆得到ScNHX1,测序得出该基因片段大小为1 617 bp,并成功构建了含该目的基因的表达载体pCAMBIA-ScNHX1。[结论]该研究为耐盐转基因植株的培育奠定了基础。[ Objective ] The aim was to clone the tonoplast Na ＋/H ＋ antiporter gene （ ScNHX1 ） and construct its expression vectors in Suaeda corniculata. [ Method] Using S. corniculata treated by 400 mmol/L NaC1 as materials, the ScNHX1 was cloned by RT-PCR technology, and the ScNHX1 was connected to the pCAMBIA1302 vector by enzyme digestion and connection methods. [ Result] The ScNHX1 was cloned suc- cessfully in the test, and the gene fragment size was 1 617 bp. The expression vector pCAMBIA-ScNHX1 containing this gene was also con- structed successfully. [ Conclusion] This study provided foundation for the breeding of salt-tolerant transgenic plants.

关 键 词：角碱蓬 液泡膜 Na+ H+逆向转运蛋白基因 

分 类 号：S332.6[农业科学—作物遗传育种]