三种不同栽培类型白芷同工酶分析  

Isozyme Analysis of Cultivated Angelicae dahuricae in Three Different Types

作　　者：曹厚强[1] 易思荣[1] 黄娅[1] 韩凤[1] 肖忠[1] 韦中强[1] 全健[1] 

机构地区：[1]重庆市药物种植研究所,重庆408435

出　　处：《安徽农业科学》2013年第3期975-976,共2页Journal of Anhui Agricultural Sciences

基　　金：重庆市科技攻关计划项目(CSTC;2012GG-YYJS0963);重庆市自然科学基金计划项目(CSTC;2012jjA10117);重庆市中医药重点攻关计划项目(2009-1-16)

摘　　要：[目的]对3种不同栽培类型的白芷进行同工酶分析。[方法]通过垂直板聚丙烯酰胺凝胶电泳技术,对选育的3种不同类型(表型)的白芷进行过氧化物酶(POD)和酯酶(EST)同工酶分析,并根据分析结果探讨其亲缘关系。[结果]过氧化物酶共得到15条谱带,其中7条是3个类型白芷的共有特征谱带;酯酶同工酶中得到12条谱带,其中6条是共有的特征谱带;聚类分析表明,3种不同类型的白芷间差异较大。[结论]该研究为白芷优良品种的选育提供了可靠且快速的生理生化选择指标。[ Objective ] The aim was to analyse the isozyme of three different types of cultivated Angelicae dahuricae. [ Method ] The peroxi- dase （POD） and esterase （EST） isozyme of three different types （phenotypes） of A. dahuricae was analyzed by the vertical slab polyacrylam- ide gel electrophoresis technique, and according to the analysis results, the genetic relationship of them was analyzed. [ Result ] A total of 15 POD and 12 EST bands were received in the test, and 7 POD and 6 EST bands of which were common characteristic bands; the cluster analy- sis showed that, A. dahurica plants in three different types varied significantly with each other. [ Conclusion] This study provided reliable and rapid physiological and biochemical index for the breeding of A. daburica.

关 键 词：白芷 过氧化物酶 酯酶 酶谱分析 

分 类 号：S567[农业科学—中草药栽培]