中华鳖和杂交鳢血清免疫球蛋白免疫印迹和酶联免疫分析  

Analysis of Immunoglobulin in the Serum of Trionyx sinensis and Ophiocephalus argus Cantor(♂)×Ophiocephalus maculatus(♀) by Western blot and ELISA

作　　者：刘凯[1] 朱丽敏[1] 蔡丽娟[1] 王宇希[1] 谢楠[1] 沈理[1] 

机构地区：[1]杭州市农业科学研究院,浙江杭州310024

出　　处：《安徽农业科学》2013年第3期1142-1144,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省科技厅公益技术研究农业项目(2011C22098);杭州市科技重大创新项目(20083212A04)

摘　　要：[目的]探讨中华鳖和杂交鳢血清免疫球蛋白的同源性及其含量。[方法]采集中华鳖和杂交鳢血清与25种商品二抗共同抚育,进行免疫印迹及酶联免疫分析。[结果]中华鳖和杂交鳢血清可与兔抗鸡IgM、兔抗猪IgG、兔抗鸡IgG、兔抗大鼠IgM、兔抗猴IgG和羊抗人IgG等6种二抗发生免疫印迹反应。利用鸡IgG、鸡IgM、猪IgG、大鼠IgM、猴IgG和人IgG的ELISA试剂盒测定了中华鳖和杂交鳢血清免疫球蛋白水平,中华鳖血清免疫球蛋白浓度为28.5～215.4μg/L,杂交鳢血清免疫球蛋白浓度为24.5～201.5μg/L。[结论]该研究能够丰富中华鳖、杂交鳢特异性免疫系统的研究,对中华鳖、杂交鳢的健康养殖有一定的指导意义。LIU Kai et al （ Hangzhou Academy of Agricultural Sciences, Hangzhou, Zhejiang 310024） Abstract [ Objective] The research aimed to study the homology and content of serum immunoglobulin in Trionyx sinensis and Ophiocephalus argus Cantor （ ♂ ） × Ophiocephalus rnaculatus（ ♀ ）. [ Method] The serum of T. sinensis and O. argus Cantor （ ♂ ） × 0. rnaculatus（ ♀ ） was sampled to cultivate with 25 commodities secondary antibodies for Western blot and ELISA analysis. [ Result] The serum of T. sinensis and O. argus Cantor （♂） × 0. maculatus（ ♀ ） reacted with six kinds of secondary antibodies（rabbit anti-chicken IgM, rabbit anti-pig IgG, rabbit anti-chicken IgG, rabbit anti-rat IgM, rabbit anti-monkey IgG and goat anti-human IgG）for Westem blot. The serum immunnoglobulin level of T. sinensis and O. argus Cantor （♂）× 0. maculatus（ ♀ ） was determined by using ELISA kits of chicken IgG,chicken IgM,swine IgG,rat IgM ,mongkey IgG and human IgG. The serum immunnoglobulin concentrations of T. sinensis and O. argus Cantor （♂） × 0. maculatus（ ♀ ） were 28.5 -215.4 and 24.5 - 201.5 μg/L respectively. [ Conclusion ] This study could enrich the specific immune system research of T. sinensis and O. argus Cantor （ ♂ ） × 0. maculatus（ ♀ ） and it was of certain guiding significance for the healthy breeding of T. sinensis and 0. argus Cantor （♂） × O. maculatus （ ♀ ）.

关 键 词：中华鳖 杂交鳢 免疫球蛋白 免疫印迹 酶联免疫 

分 类 号：S947.1[农业科学—水产养殖]