梭鱼养殖群体与自然群体的遗传结构研究  

Studies on the Genetic Structure of Cultivated Population and Natural Population of Liza haematocheila

作　　者：王茜[1] 边靖 

机构地区：[1]天津农学院水产科学系天津市水产生态及养殖重点实验室,天津300384 [2]天津静海县水产良种场,天津301600

出　　处：《安徽农业科学》2013年第3期1145-1147,共3页Journal of Anhui Agricultural Sciences

基　　金：天津自然科学基金(11JCYBJC08200);天津市高等学校科技发展基金项目

摘　　要：[目的]掌握渤海地区梭鱼种群的种质资源状况。[方法]应用随机扩增多态DNA(RAPD)技术,对梭鱼养殖群体与自然群体的遗传多样性进行了分析。[结果]从45个随机引物中选出20个扩增效果稳定的引物进行群体分析。其中,梭鱼自然群体共检出了204个扩增位点,多态位点136个,多态位点比例为66.7%;养殖群体中共检出了217个扩增位点,多态位点130个,多态位点比例为59.9%。[结论]目前梭鱼群体的遗传多样性较为丰富,人工养殖还未对其造成明显的影响。[ Objective] The research aimed to study the resources of Liza haematocheila population in Bohai Region. [ Method ] Using Ran- dom amplified polymorphic DNA （RAPD）, the genetic diversity of cultivated population and natural population of L. haematocheila was ana- lyzed. [ Result] 20 primers with stable amplification effects were selected from 45 random primers for population analysis. In natural popula- tion, 204 amplification sites were detected, and 136 sites were polymorphic and the percentage of polymorphic loci was 66.7%. In cultivated population, 217 amplification sites were detected, and 130 sites were polymorphic and the percentage of polymorphic loci was 59.9%. [ Con- clusion] At present, L. haematocheila oor）ulation has abundant genetic diversity and artificial cuhivation hasn＇ t obvious effects.

关 键 词：梭鱼 随机扩增多态DNA 遗传多样性 养殖群体 自然群体 

分 类 号：S965.234[农业科学—水产养殖]