牛大力多糖含量的测定  

Determination of Polysaccharide Content in Millettia speciosa Champ.

作　　者：黄江燕[1] 唐专辉[2] 李典鹏 温艳蓉[2] 朱艺萍[2] 莫静[2] 罗轩[2] 

机构地区：[1]广西代谢性疾病研究重点实验室中国人民解放军第181医院,广西桂林541002 [2]广西大学化学化工学院,广西南宁530004 [3]广西植物研究所广西植物功能物质研究与利用重点实验室,广西桂林541006

出　　处：《安徽农业科学》2013年第3期1180-1180,1195,共2页Journal of Anhui Agricultural Sciences

基　　金：广西代谢性疾病研究重点实验室开放基金课题(181H2011-02);广西植物功能物质研究与利用重点实验室开放基金课题(FPRU2011-4);广西大学"大学生创新创业训练计划"项目(1201037)

摘　　要：[目的]测定牛大力多糖含量,为牛大力抗疲劳活性研究提供依据。[方法]采用苯酚–硫酸法测定牛大力醇提物中水溶性成分多糖的含量。[结果]该试验方法在1～9μg/ml浓度范围内具有线性关系,稳定性和精密度良好,加样回收率为103.4%,测得牛大力多糖含量为62.3%。[结论]牛大力多糖作为抗疲劳活性的主要成分之一,具有进一步研究的价值。[ Objective ] The polysaccharide content of MiUettia speciosa Champ. was determined to provide the data for anti-fatigue research. [ Method] Phenol-sulfuric acid method was used to determine the content of polysaccharide in M. speciosa. [ Result] The method had good linearity in the concentration of 1 -9 ~g/ml. The stability and precision were also good. The recovery rate was 103.4%. The total amount of polysaccharides in M. speciosa was 62.3%. [ Conclusion] The polysaccharides of M. speciosa maybe one of components of anti-fatigue activity and is worth for further study.

关 键 词：牛大力 多糖 含量测定 抗疲劳 

分 类 号：S567[农业科学—中草药栽培]