甘草酸二铵对溃疡性结肠炎大鼠M30表达的影响  

Treatment with diammonium glycyrrhizinate down-regulates M30 expression in ulcerative colitis in rats

作　　者：原皓[1] 王鹤鸣[1] 赵雪曼[1] 

机构地区：[1]潍坊医学院临床学院,山东省潍坊市261042

出　　处：《世界华人消化杂志》2012年第35期3439-3444,共6页World Chinese Journal of Digestology

基　　金：山东省自然科学基金资助项目;No.ZR2010HM089~~

摘　　要：目的:观察甘草酸二铵(diammonium glycyrrhi-zinate,DG)对2,4,6-三硝基苯磺酸(2,4,6-trin-itrobenzene sulfonic acid,TNBS)诱导的大鼠溃疡性结肠炎(ulcerative colitis,UC)的疗效及其对大鼠结肠组织M30及Fas/FasL蛋白表达的影响,继而从细胞凋亡方面探讨DG治疗大鼠UC可能的作用机制.方法:♀Wistar大鼠30只,随机分为正常对照组、模型组和DG组,每组10只.用TNBS/乙醇灌肠法复制大鼠UC模型,10d后收集结肠标本,观察大鼠疾病活动指数(disease activity index,DAI)、结肠大体形态损伤评分和组织学改变,用免疫组织化学法观察大鼠结肠黏膜中M30及Fas/FasL蛋白的表达.结果:与正常对照组相比,DG组、模型组DAI评分、结肠大体形态损伤评分和组织学损伤评分均显著增高(7.06±0.80vs0.32±0.14;6.03±0.61vs0.19±0.16;5.84±0.53vs0.22±0.11,P<0.01);而与模型组相比,DG治疗组能显著改善UC症状(3.33±0.27vs7.06±0.80;3.29±0.36vs6.03±0.61;2.98±0.24vs5.84±0.53,P<0.05).模型组大鼠M30及Fas/FasL蛋白表达水平明显高于正常组(5.76±0.66vs0.42±0.18;26.62±4.20vs10.81±2.20;17.11±3.12vs6.02±1.02,P<0.01);与模型组相比,DG治疗组M30及Fas/FasL蛋白表达显著降低(2.24±0.48vs5.76±0.66;17.23±3.20vs26.62±4.20;11.02±2.12vs17.11±3.12,P<0.05).结论:DG对TNBS诱导的大鼠UC有较好的疗效,DG通过下调Fas/FasL的表达抑制结肠上皮细胞的凋亡,这可能是其减轻结肠黏膜损伤的机制之一.AIM：To assess the therapeutic effect of diammonium glycyrrhizinate （DG） on 2,4,6-trinitrobenzene sulfonic acid （TNBS）-induced ulcerative colitis in rats and to explore the underlying mechanisms by detecting the expression of M30 and Fas/FasL. METHODS： Thirty Wistar rats were equally randomized into a normal control group, a model group and a DG group. Ulcerative colitis was induced in the DG group and model group with TNBS by enema. After ten days, all rats were killed. Disease activity index （DAI）, colon macroscopic damage score （CMDS） and histological damage score were calculated, and the expression of M30 and Fas/FasL in the colonic mucosa was detected by immunohistochemistry. RESULTS：Compared to normal controls, the DAI, CMDS and histological damage score significantly increased in model rats （7.06 ± 0.80 vs 0.32 ± 0.14; 6.03 ± 0.61 vs 0.19 ± 0.16; 5.84 ± 0.53 vs 0.22 ± 0.11, P 0.01）. Compared to the model group, the above parameters were significantly improved in the DG group （3.33 ± 0.27 vs 7.06 ± 0.80; 3.29 ± 0.36 vs 6.03 ± 0.61; 2.98 ± 0.24 vs 5.84 ± 0.53, P 0.05）. Compared to the normal control group, the expression of M30 and Fas/FasL was up-regulated in the model group and DG group （5.76 ± 0.66 vs 0.42 ± 0.18; 26.62 ± 4.20 vs 10.81 ± 2.20; 17.11 ± 3.12 vs 6.02 ± 1.02, P 0.01）. Compared to the model group, the expression of M30 and Fas/FasL was remarkably decreased in the DG group （2.24 ± 0.48 vs 5.76 ± 0.66; 17.23 ± 3.20 vs 26.62 ± 4.20; 11.02 ± 2.12 vs 17.11 ± 3.12, P 0.05）. CONCLUSION：Treatment with DG could reduce in？ammatory injury in rats with experimental ulcerative colitis possibly by reducing the expression Fas/FasL and inhibiting apoptosis of cells in the colonic mucosa.

关 键 词：溃疡性结肠炎 甘草酸二铵 FAS FASL M30 细胞凋亡 

分 类 号：R965[医药卫生—药理学]