单中心191例健康供者应用G-CSF动员造血干细胞的影响因素分析  

Analysis of Influence Factors on G-CSF-Mobilized Hematopoietic Stem Cells of 191 Healthy Donors in a Monocenter

作　　者：王倩[1,2] 袁磊[1] 李红华[1] 赵瑜[1] 黄文荣[1] 薄剑[1] 王书红[1] 朱海燕[1] 窦立萍[1] 王全顺[1] 于力[1] 靖彧[1] 

机构地区：[1]解放军总医院血液科,北京100853 [2]吉林省长春市一汽总医院血液肿瘤科,吉林长春130011

出　　处：《中国实验血液学杂志》2013年第1期169-172,共4页Journal of Experimental Hematology

基　　金：解放军总医院苗圃基金(编号07MP03)

摘　　要：本研究旨在探讨粒细胞集落刺激因子(G-CSF)动员外周血造血干细胞的影响因素及对健康供者的影响。181例志愿健康供者应用G-CSF 5-10μg/(kg.d)动员,10例应用G-CSF 3.3-4.9μg/(kg.d)动员,12 h 1次,连续动员4-5 d;采集、检测外周血中单个核细胞(MNC)数与CD34+细胞数,并观察供者动员及采集过程中的不良反应。结果表明,与动员前相比,动员后(采集前)供者外周血白细胞数平均升高7倍(P<0.01);血小板数明显下降(P<0.01);血红蛋白含量无明显差异性。动员第4或5天采集效果无差异。男性供者采集的MNC、CD34+细胞数高于女性(P<0.01),高体重供者采集的MNC、CD34+数高于低体重供者,年龄对采集效果无明显影响。G-CSF剂量与采集效果无线性关系。供者不良反应轻微。结论:G-CSF可以有效动员外周血造血干细胞,供者无明显的不良反应。This study was aimed to investigate the factors influencing mobilization efficiency of peripheral hematopoi- etic stem cells with granulocyte colony stimulating factor （G-CSF） and their impact on healthy donors. 181 donors were mobilized subcutaneously with G-CSF at 5 -10 μg/（kg·d）, and 10 donors were mobilized with G-CSF at 3.3 -4.9μg/（kg·d）, once 12 h, for4 -5 d. Peripheral blood mononuclear cell （MNC） and CD34＋cell counts were analyzed by flow cytometry. Mobilization-related side effects were also monitored. The results showed that white blood cell counts increased by 6 times averagely after mobilization （ P 〈 0.01 ）. The platelet count obviously decreased （ P 〈 0. 01 ）, while the hemoglobin level did not show significant difference. No significant differences were observed in MNC and CD34 ＋ cell counts between those subjects harvested on the 4th and 5th day after mobilization. Male donors were superior to female ones in cell harvest（ P 〈 0.01 ）. Donor body weight played positive role in cell yield, while impact of age on harvest was not remarkable. Neither MNC nor CD34 ＋ cell count showed a linear relationship with G-CSF dose. Only slight side effects were observed on the donors in this study. It is concluded that mobilization with G-CSF is sufficient in healthy donors without remarkable side effects.

关 键 词：粒细胞集落刺激因子 动员 供者 外周血造血干细胞 

分 类 号：R331.2[医药卫生—人体生理学] R973.4[医药卫生—基础医学]