α-萜品醇熏蒸对大麦虫体内抗氧化酶活性的影响  

Effects of α-terpineol fumigation on the in vivo antioxidant activity in Zophobas morio (Coleoptera: Tenebrionidae)

作　　者：田雨浓[1] 马伟[2] 韦庆慧[3] 罗帅[1] 韩旭洋[1] 陈旭日[1] 邱本军[1] 马玲[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]黑龙江中医药大学药学院,哈尔滨150040 [3]东北林业大学生命科学学院,哈尔滨150040

出　　处：《昆虫学报》2013年第1期47-53,共7页Acta Entomologica Sinica

基　　金：林业公益性行业专项(201104069);黑龙江省科技攻关项目(GA09B203-1)

摘　　要：为研究植物挥发性有机化合物α-萜品醇的杀虫活性及作用机理,本研究采用熏蒸法测定了α-萜品醇对大麦虫Zophobasmorio(鞘翅目:拟步行甲科)4龄幼虫的急性毒性,并测定了不同熏蒸时间后幼虫体内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性。结果表明:熏蒸48h时,α-萜品醇对大麦虫4龄幼虫的LC50和LC20值分别为69.425μg/L和59.916μg/L。α-萜品醇(LC20和LC50)处理的4龄幼虫SOD,POD和CAT活性均表现为先升高后降低的趋势。据此推测,α-萜品醇在幼虫体内积累显著影响幼虫体内SOD,POD和CAT活性,降低虫体内自由基的清除能力,从而对其产生毒害作用。In order to study the insecticidal activity and toxicity mechanism of α-terpineol, a plant volatile organic compound, we tested the acute toxicity of α-terpineol against the 4th instar larvae of Zophobas morio in a gas-fumigation system and investigated the in vivo activities of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） in the treated larvae at different fumigation duration. The results showed that the LC50 and LC20 values of α-terpineol against the 4th instar larvae in acute toxicity test at 48 h after fumigation were 69. 425μg/L and 59. 916 μg/L, respectively. Under the fumigation of α-terpineol at two concentrations （ LC50 and LC20 ） , the activities of SOD, POD and CAT showed a time-course change, first increasing and then decreasing. It is so inferred that the gradual accumulation of α-terpineol in the treated larvae produces significantly negative effects on the activities of SOD, POD and CAT in their bodies, and this may lead to a decline in scavenging activity of free radicals and stronger toxic effects on Z. morio larvae.

关 键 词：大麦虫 α-萜品醇 熏蒸 急性毒性 保护酶 酶活性 

分 类 号：Q965[生物学—昆虫学]