阻断交感神经对青年和老龄小鼠小肠T淋巴细胞活性的影响  

Effects of sympathetic nerve on the activity of intestinal T lymphocyte in young and aged mice

作　　者：柯妍妍[1] 朱琪[2] 刘文杰[3] 林锦清[2] 李清[2] 王娟 

机构地区：[1]厦门医学高等专科学校微生物学与免疫学教研室,361008 [2]福州市第二医院肝胆内科,350007 [3]厦门大学药学院,361005 [4]山东省平度市第三人民医院内科,266753

出　　处：《免疫学杂志》2013年第3期195-199,共5页Immunological Journal

基　　金：福州市科技司[榕科2011(106)号]

摘　　要：目的探究交感神经在青年和老龄小鼠中对小肠T淋巴细胞的免疫调控机制。方法选用健康的3月龄和18月龄的雄性昆明小鼠,腹腔注射六羟多巴胺,通过石蜡切片和HE染色观察上皮内淋巴细胞的数量,MTT法测定小肠T淋巴细胞的增殖,ELISA试剂盒测定小肠T淋巴细胞分泌IL-2、IL-4水平。结果阻断交感神经后,2个年龄组上皮内淋巴细胞数量均减少,老龄组尤为突出。青年小鼠T淋巴细胞增殖在处理后第5、10天出现下降,老龄小鼠在第3天至第15天均显著下降,其中第5天下降39.4%(P<0.01)。青年小鼠损毁交感神经后主要引起淋巴细胞分泌IL-4含量降低,而老龄小鼠主要引起IL-2降低。结论损毁交感神经可导致青年和老龄小鼠小肠T淋巴细胞活性下降,老龄组下降幅度大,恢复周期长。We aimed to investigate the effect of sympathetic nerve on intestinal T lymphocyte activity of adult and aged mice. The healthy 3-month-old and 18-month-old mice were sued to construct sympathectomy mousemodel by injecting 6-OHDA intraperitoneally. The number of intraepithelial lymphocytes （IEL）, the T lymphocyte proliferation and the production of both IL-2 and IL-4 were studied by HE staining, MTT, and ELISA methods,respectively. The results showed that the numbers of IEL were decreased in both of young and aged mice, especially in the aged mice. In young mice, the T lymphocyte proliferation decreased at 5^th and 10^th days after 6-OHDAinjection; in aged mice, the T lymphocyte proliferation decreased from the 3^rd day to the 5^th day （decreased by 39.4%, P〈 0.01） after 6-OHDA injection. And the IL-4 production in the lymphocyte supernatant decreased mainlyin young treated mice, but the IL-2 production decreased mainly in aged treated mice. The results suggested that sympathectomy could reduce the activity of intestinal T lymphocyte in both young and aged mice. The activitydecrease is more obvious and the recovery period is longer in aged mice.

关 键 词：交感神经 年龄 T淋巴细胞 小肠 小鼠 

分 类 号：R392.1[医药卫生—免疫学]