LPS通过TLR4信号通路诱导人肾小管上皮细胞株(HK-2)表达hBD-2  

Effects of LPS-TLR4 signaling pathway on the expression of hBD-2 in human tubular epithelial cells

作　　者：赵俊丽[1] 王俭勤[1] 王晶宇 

机构地区：[1]兰州大学第二医院肾病科,730030 [2]基础医学院病理生理学研究所

出　　处：《免疫学杂志》2013年第3期222-225,共4页Immunological Journal

基　　金：国家自然科学基金项目(81141057)

摘　　要：目的研究LPS体外刺激人肾小管上皮细胞株(HK-2)是否诱导表达hBD-2,并进一步探讨该表达与TLR4/NF-κB信号传导通路的关系。方法给予不同质量浓度的LPS(0.01、0.1、1、10μg/ml)刺激HK-2细胞12 h,再应用TLR4及NF-κB阻断剂预处理HK-2细胞1 h后,给予质量浓度为1μg/ml的LPS作用12 h,采用实时荧光定量PCR检测HK-2细胞hBD-2 mRNA的表达,ELISA检测细胞上清中hBD-2的表达。结果 1)LPS能诱导HK-2细胞hBD-2 mRNA及蛋白的表达,且这种表达具有质量浓度依赖性;2)TLR4阻断剂能抑制LPS对HK-2细胞表达hBD-2的诱导作用,与未阻断之前相比差异具有统计学意义(P<0.01);3)NF-κB阻断剂能抑制LPS对HK-2细胞表达hBD-2的诱导作用,与未阻断之前相比差异具有统计学意义(P<0.01)。结论 LPS可能通过TLR4/NF-κB信号传导通路诱导HK-2细胞表达hBD-2,将为泌尿系统感染防治提供新靶点。We aimed to identify whether lipopolysaccharide （LPS） could up-regulate the expression of human beta-defensin-2 （hBD-2） in human tubular epithelial eell （HK-2）, and further discuss the role of TLR4/NF-κBsignaling pathways in this procedure. HK-2 cells were stimulated with LPS at concentrations of 0.01, 0.1, 1, 10 p.g/ ml respectively, or pretreated with TLR4 or NF-κB blocker and then stimulated with 1 ug/ml LPS respectively.12 hours later, we detected the expression of hBD-2 mRNA by SYBR using Green fluorescent quantitative realtime PCR in HK-2 cells. And the concentration of hBD-2 in supernatants was measured by ELISA. We found thatLPS promoted hBD-2 expression in HK-2 cells in a dose-dependent manner. Otherwise, TLR4 and NF-κB blockers could inhibit the LPS-induced hBD-2 expression in HK-2 cells. We concluded that LPS can induce hBD-2 expression in HK-2 cells by TLR4/NF-κB signaling pathways in a dose-dependent manner. This founding will benefit the therapy for urinary tract infection.

关 键 词：人肾小管上皮细胞 人B防御素-2 TOLL样受体4 脂多糖 NF-κB 

分 类 号：R692[医药卫生—泌尿科学]