利用二次谐波成像技术监测成兔耳皮肤创伤愈合和瘢痕形成过程  

Monitor Ear Skin Wound Healing and Scarring Process of Adult Rabbit with Second Harmonic Generation Imaging Technique

作　　者：熊舒原[1] 曹宁[2] 黄祖根[1] 汤逸颜[1] 郭春根[1] 

机构地区：[1]福建医科大学附属第一医院整形外科,福州市350004 [2]福建医科大学附属漳州市医院

出　　处：《中国激光医学杂志》2013年第1期5-9,64,共6页Chinese Journal of Laser Medicine & Surgery

基　　金：国家自然科学基金(30970783);开放课题(JYG1206);福建省卫生厅青年科研课题(20102122)

摘　　要：目的探讨基于二次谐波信号的光学成像技术监测兔耳创伤愈合及瘢痕形成过程。方法选用新西兰大耳白兔6只,在兔耳腹侧面中部建立8 mm的圆形皮肤创伤模型,定期取材,用二次谐波成像技术观察兔耳皮肤创伤愈合的动态变化过程。结果在兔耳创伤愈合过程中,兔耳腹侧创面产生增生性瘢痕,其新生胶原在二次谐波成像仪下产生二次谐波信号,于创伤早期出现纤细的胶原,随着创伤愈合的进展,胶原含量逐渐增多至瘢痕形成高峰期,后随着胶原组织的重塑,胶原部分降解,胶原含量略减少且逐渐与表皮平行排列。结论二次谐波成像技术可以定量表征创伤愈合不同阶段的形态特征,有望用于在体监测活体生物组织创伤愈合及瘢痕形成过程,这将在临床医学应用中具有重大的研究意义和广阔的应用前景。Objective To explore the role of optical imaging technique based on second harmonic generation （SHG） in monitoring the healing and scarring processes of rabbit ear wounds. Methods Six white adult New Zealand male rabbits were selected for the experiment. The epidermis and perichondrium on the ventral surface of their ears were removed to create round wounds with a diameter of 8 mm. The samples were collected at regular intervals to observe the dynamic changes and wound healing and scarring processes with nonlinear spectral imaging technology. Results The new tissues produced SHG signals. Collagen content and microstructure differed dramatically at different time points a-long the wound healing and scarring course. Hypertrophic scars were found on the ventral side of the rabbit ears, and slender collagen appeared in the early stage of the wounding. The content of the collagen increased gradually as the wound was healing. With the remod- eling of the collagen, some of the collagen was degraded, the content dropped slightly and the collagen became parallel to the epidermis gradually. Conclusions Second harmonic generation imaging technique can be used to observe sample slices, which is useful for quantitative characterization of wound status during wound healing and scarring processes. Hopefully, it will be used to observe wound healing and scarring processes in vivo, which will be of great significance to clinical research with bright prospect in clinical application.

关 键 词：激光 二次谐波成像技术 兔耳皮肤增生性瘢痕模型 胶原 

分 类 号：R622[医药卫生—整形外科]