Assessment of a five-color flow cytometric assay for verifying automated white blood cell differentials  

作　　者：HUANG Chun-mei YU Lian-hui PU Cheng-wei WANG Xin WANG Geng SHEN Li-song WANG Jian-zhong CUI Wei 

机构地区：[1]Department of Clinical Laboratory, Chinese Academy of Medical Sciences & Peking Union Medical College Hospital, Beijing 100730, China [2]Department of Clinical Laboratory, Peking University First Hospital, Beijing 100034, China [3]Department of Clinical Laboratory, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China

出　　处：《Chinese Medical Journal》2013年第4期716-721,共6页中华医学杂志（英文版）

摘　　要：Background White blood cell （WBC） counts and differentials performed using an automated cell counter typically require manual microscopic review. However, this last step is time consuming and requires experienced personnel. We evaluated the clinical efficiency of using flow cytometry （FCM） employing a six-antibody/five-color reagent for verifying automated WBC differentials. Methods A total of 56 apparently healthy samples were assessed using a five-color flow cytometer to verify the normal reference ranges of WBC differentials. WBC differentials of 622 samples were also determined using both a cell counter and FCM. These results were then confirmed using manual microscopic methods. Results The probabilities for all of the parameters of WBC differentials exceeded the corresponding normal reference ranges by no more than 7.5%. The resulting WBC differentials were well correlated between FCM and the cell counter （r 〉0.88, P 〈0.001）, except in the case of basophils. Neutrophils, lymphocytes, and eosinophils were well correlated between FCM and standard microscopic cytology assessment （r 〉0.80, P 〈0.001）. The sensitivities of FCM for identification of immature granulocytes and blast cells （72.03% and 22.22%, respectively） were higher than those of the cell counter method （44.92% and 11.11%, respectively）. The specificities of FCM were all above 85%, substantially better than those of the cell counter method. Conclusion These five-color FCM assays could be applied to accurately verify abnormal results of automated assessment of WBC differentials.Background White blood cell （WBC） counts and differentials performed using an automated cell counter typically require manual microscopic review. However, this last step is time consuming and requires experienced personnel. We evaluated the clinical efficiency of using flow cytometry （FCM） employing a six-antibody/five-color reagent for verifying automated WBC differentials. Methods A total of 56 apparently healthy samples were assessed using a five-color flow cytometer to verify the normal reference ranges of WBC differentials. WBC differentials of 622 samples were also determined using both a cell counter and FCM. These results were then confirmed using manual microscopic methods. Results The probabilities for all of the parameters of WBC differentials exceeded the corresponding normal reference ranges by no more than 7.5%. The resulting WBC differentials were well correlated between FCM and the cell counter （r 〉0.88, P 〈0.001）, except in the case of basophils. Neutrophils, lymphocytes, and eosinophils were well correlated between FCM and standard microscopic cytology assessment （r 〉0.80, P 〈0.001）. The sensitivities of FCM for identification of immature granulocytes and blast cells （72.03% and 22.22%, respectively） were higher than those of the cell counter method （44.92% and 11.11%, respectively）. The specificities of FCM were all above 85%, substantially better than those of the cell counter method. Conclusion These five-color FCM assays could be applied to accurately verify abnormal results of automated assessment of WBC differentials.

关 键 词：flow cytometry white blood cell count HEMATOLOGY manual microscopy 

分 类 号：R446.1[医药卫生—诊断学]