α-突触核蛋白和葡糖脑苷脂酶在食蟹猴不同脑区的表达  

Differential expressions of α-synuclein and glucocerebrosidase in different brain regions of cynomolgus monkeys

作　　者：弥娜[1] 刘光伟[1] 李昕[1] 李尧华[1] 于顺[1] 

机构地区：[1]首都医科大学宣武医院神经生物学研究室北京市老年病医疗研究中心分子诊断实验室教育部神经变性病重点实验室,北京100053

出　　处：《首都医科大学学报》2013年第1期110-114,共5页Journal of Capital Medical University

基　　金：国家重点基础发展计划(973项目)(2011CB504101);国家自然科学基金(81071014);国家高技术研究发展计划(863计划)(2006AA02A408);北京市自然科学基金(7102076);北京市属高等学校人才强教计划资助项目(PHR200907113)~~

摘　　要：目的研究α-突触核蛋白(α-synuclein,α-Syn)及葡糖脑苷脂酶(glucocerebrosidase,GCase,GBA)在食蟹猴不同脑区的表达。方法 Western blotting方法及免疫组织化学法检测α-Syn和GBA在食蟹猴纹状体和小脑皮质的表达。酶活性分析法检测GBA活性。免疫荧光双重标记法观察α-Syn和GBA的共定位。结果α-Syn和GBA在胞质和神经末梢中存在共定位。α-Syn、GBA在纹状体和小脑部位均有表达,但α-Syn在纹状体表达量高于小脑,而GBA在纹状体表达量低于小脑。此外,纹状体的GBA酶活性低于小脑。结论不同脑区α-Syn、GBA表达量不同,α-Syn表达量与GBA表达量呈反向关系。Objective To investigate the expressions of α-synuclein （α-Syn） and glucocerebrosidase （GBA） in different brain regions of cynomolgus monkeys. Methods Western blotting and immunohistochemical methods were applied to measure the levels of α-Syn and GBA in striatum and cerebellum of cynomolgus monkeys. Enzyme activity assay was used to determine GBA activity. Double immunofluorescent labeling was used to examine the co-localization of α-Syn and GBA. Results α-Syn and GBA were colocalized in neuronal cytoplasm and nerve terminals. α-Syn and GBA were expressed in both the striatum and cerebellum. However, the level of α-Syn in striatum was higher than that in cerebellum while the level of GBA in striatum was less than that in cerebellum. The activity of GBA was also lower in striatum than in cerebellum. Conclusion The expression levels of α-Syn and GBA are different in different brain regions. The level of α-Syn is negatively correlated with that of GBA.

关 键 词：Α-突触核蛋白 葡糖脑苷脂酶 食蟹猴 

分 类 号：R338[医药卫生—人体生理学]