氯胺酮对脂多糖所致大鼠抑郁样症状的影响  

Effects of ketamine on lipopolysaccharide-induced depression-like symptoms in rats

作　　者：杨春[1] 胡婷婷[1] 沈江[1] 洪涛[1] 周志强[2] 胡益民[2] 

机构地区：[1]常州市第一人民医院麻醉科,江苏省213003 [2]南京军区南京总医院麻醉科

出　　处：《江苏医药》2013年第4期379-380,共2页Jiangsu Medical Journal

基　　金：国家自然科学基金(30872424);全军"十二五"医药卫生科研基金(CWS11J017)

摘　　要：目的探讨氯胺酮对脂多糖所致大鼠抑郁样症状的影响。方法 30只雄性Wistar大鼠随机均分为对照(C)组、生理盐水(S)组和氯胺酮(K)组。K组及S组大鼠腹腔注射1mg/kg脂多糖,24h后分别注射10mg/kg氯胺酮和等体积生理盐水。30min后,三组大鼠行强迫游泳实验6min,记录后5min不动时间。立即处死大鼠,取前额皮层采用ELISA法测定IL-1β和IL-6的含量。结果与C组相比,S组大鼠强迫游泳不动时间显著延长(P<0.01),且前额皮层IL-1β及IL-6表达显著上调(P<0.05);与S组相比,K组大鼠强迫游泳不动时间显著减少,且前额皮层IL-1β及IL-6表达显著下调(P<0.05)。结论氯胺酮能显著改善脂多糖所致大鼠抑郁样症状,可能与下调前额皮层IL-1β及IL-6表达有关。Objective To investigate the effects of ketamine on lipopolysaccharide-induced depression-like symptoms in rats.Methods Thirty male Wistar rats were equally randomized into 3 groups of C（control group）,S（saline group） and K（ketamine group）.Lipopolysaccharide 1 mg/kg was intraperitoneally injected in the rats of groups of K and S,which were given ketamine 10 mg/kg or same volumn of saline 24 hours later,respectively.Thirty minutes later,forced swimming test（FST） was carried out in 3 groups and the immobility time during FST was recorded.Subsequently,all rats were sacrificed and prefrontal cortex was harvested for the determinations of IL-1β and IL-6 by ELISA.Results Compared with group C,the immobility time of FST was longer（P0.01） and expressions of IL-1β and IL-6 in prefrontal cortex were higher（P0.05） in group S.Compared with group S,the immobility time of FST was shorter and expressions of IL-1β and IL-6 in prefrontal cortex were lower in group K（P0.05）.Conclusion Ketamine is able to ameliorate lipopolysaccharide-induced depression-like symptoms in rats,which is probably related to downregulation of IL-1β and IL-6 in the prefrontal cortex.

关 键 词：氯胺酮 抑郁 白细胞介素1Β 白细胞介素6 

分 类 号：R965[医药卫生—药理学]