枸杞多糖对β-TC6细胞胰岛素分泌及相关基因的影响  

Effects of lycium barbarum polysaccharide on insulin secretion and its related gene expression in β-TC6 cells

作　　者：蔡慧珍[1,2] 刘福康[1] 孙桂菊[1] 

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室,江苏省210009 [2]宁夏医科大学

出　　处：《江苏医药》2013年第4期391-393,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金项目(30872119);高等学校博士学科点专项科研基金(20100092110039)

摘　　要：目的研究枸杞多糖(LBP)对小鼠胰岛β-TC6细胞胰岛素分泌及相关基因的影响。方法用浓度为0、12.5、25、50、100、200、300μg/ml的LBP处理β-TC6细胞,MTT法检测细胞增殖活性,ELISA法检测LBP对葡萄糖刺激胰岛素的分泌水平,RT-PCR法检测胰岛素及相关基因mRNA表达。结果 LBP呈浓度依赖性地刺激β-TC6细胞生长。在葡萄糖浓度为20mmol/L的高糖环境下,LBP≥25μg/ml时胰岛素分泌明显增多(P<0.01)。LBP 12.5μg/ml、100μg/ml能显著降低胰岛素mRNA表达(P<0.01),但对胰十二指肠同源盒因子1、葡萄糖激酶和葡萄糖转运蛋白2基因mRNA表达无明显影响。结论 LBP的促胰岛素分泌作用可能与促进β-TC6细胞增殖有关,而与胰岛素相关基因表达无关。Objective To investigate the effects of lycium barbarum polysaccharide（LBP） on the insulin secretion and its related gene expression in β-TC6 cells.Methods β-TC6 cells were treated with different concentrations of LBP 0,12.5,25,50,100,200,300 μg/ml in vitro.The proliferation of β-TC6 cells and the level of LBP-induced insulin secretion under higher glucose environment were measured by MTT and ELISA,respectively.The mRNA expressions of insulin and its related genes were detected by RT-PCR.Results The growth of β-TC6 cells induced by LBP was increased in a dose-dependent manner（P0.01）.The insulin secretion stimulated with LBP≥25 μg/ml was significantly increased under high glucose environment（glucose 20 mmol/L）.After β-TC6 cells were treated with LBP 12.5 and 100 μg/ml（P0.01）,the mRNA expression of insulin was obviously decreased,but the mRNA expressions of pancreatic-duodenal homeobox-1,glucokinase and glucose transporter-2 were not remarkably changed.Conclusion LBP-promoting insulin secretion may result from the increase of cell proliferation,but is not relevant to the mRNA expression of insulin-related genes.

关 键 词：枸杞多糖 胰岛素 胰十二指肠同源盒因子1 葡萄糖激酶 葡萄糖转运蛋白2 

分 类 号：R994[医药卫生—毒理学]