姜黄素对直肠癌CDl33＋肿瘤干细胞样细胞群体放疗增敏作用的研究  

The radiosensitizing effect of curcumin on CD133 ＋ rectal cancer cells

作　　者：王幸[1] 裘建明[2] 杨关根[1] 陆新建[1] 封巍[1] 沈忠[1] 

机构地区：[1]北京市怀柔区中医医院普外科,101400 [2]杭州市第三人民医院肛肠外科

出　　处：《中华普通外科杂志》2013年第2期134-137,共4页Chinese Journal of General Surgery

基　　金：浙江省自然科学基金资助项目（Y2110782）;浙江省科技厅公益技术研究社会发展资助项目（2012C33SAl00045）;浙江省医药卫生平台骨干计划资助项目（2011RCA035）;国家自然科学青年基金资助项目（81101876/H1617）

摘　　要：目的探讨姜黄素对体外、体内直肠癌CDl33＋肿瘤干细胞样细胞群放疗敏感性的影响。方法采用免疫磁珠分选法对直肠癌HRT-18细胞进行干细胞分选后随机分成姜黄素组、放疗组、姜黄素联合放疗组，应用Mrrr法检测24、48和72h各组细胞生长抑制情况；采用AnnexinV／PI双染流式细胞仪检测各组细胞凋亡情况。将分选的CDl33’直肠癌细胞移植于裸鼠右脚垫建立移植瘤模型，将模型鼠分为姜黄素组、放疗组、姜黄素联合放疗组，每组8只，观察瘤体动态生长情况并用原位末端标记技术检测瘤体内细胞凋亡情况。结果低剂量姜黄素联合放疗对直肠癌CDl33’肿瘤干细胞样细胞群（24h生长抑制率：18．7％±1．7％）较放疗组（14．6％±1．0％）具有明显的生长抑制作用（P〈0．叭）；姜黄素联合放疗组细胞凋亡率（28．8％±3．7％）与放疗组（13．1％．4-1．4％）比较差异有统计学意义（P〈0．01）；治疗6d时，姜黄素联合放疗组的瘤体体积为（521±79）mm’，与放疗组的（717±134）mm。比较差异有统计学意义（P〈0．01）；姜黄素联合放疗组瘤体细胞凋亡指数（26．1％,4-3．3％）较放疗组（12．0％±2．1％）明显增高，两者比较差异有统计学意义（P〈0．01）。结论姜黄素可显著提高CDl33’肿瘤干细胞样细胞群的放疗敏感性。Objective To investigate the effect of curcumin an extract of a Chinese medical herb on the sensitivity of CD133＋ rectal cancer cells to radiotherapy. Methods In vitro experiments ： CD133 ＋ cells were purified with immunomagnetic beads from HRT-18 cell line and divided into curcumin group, radiotherapy group and curcumin plus radiotherapy group. M3T assay and Annexin V/PI staining were used to measure the proliferation and apoptosis of the cells. In vivo experiments： Transplanted rectal tumor was established in 46 nude mice and randomly divided into curcumin group, radiotherapy group and curcumin plus radiotherapy group. Tumor size and apoptosis were detected by daily observation and TUNEL staining respectively. Results Curcumin inhibited proliferation and apoptosis of CD133 ＋ rectal cancer cells when combined with radiotherapy. It also significantly increased the growth inhibition of rectal tumor and promoted the apoptosis of rectal cancer in vivo. M3T assay showed that after 24 hours, compared with that of radiotherapy group（ 14. 6% ＋ 1.0% ） , curcumin plus radiotherapy group（ 18.7% -＋ 1.7% ） inhibited the growth of the tumor（ P 〈 0. 01 ）. Annexin V/PI showed that curcumin plus radiotherapy group（ 28.8%o _＋ 3.7% ） was significantly different from the radiotherapy group（ 13.1%o ＋-1.4% ） in cell apoptosis （P 〈 0. 01）. In vivo, after 6 days, tumor volume （521 ＋_ 79） mm3 in curcumin plus radiotherapy group was significantly lower than that of radiotherapy group （717 ＋ 134 ）mm3 （P 〈 0. 01 ） ; TUNEL staining results indicated that the RCST in curcumin plus radiotherapy group （26. 1% ＋- 3.3%o ） were higher than that in radiotherapy group （ 12. 0% ＋ 2. 1% ） （ P 〈 0. 01 ）. Conclusions Curcumin significantly enhances the radiosensitizing effect for CD133 ＋ rectal cancer cells.

关 键 词：直肠肿瘤 姜黄素 肿瘤干细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R735.37[医药卫生—中医学]