用单因子血清识别急性致瘤性ALV诱发肿瘤组织中的fps肿瘤抗原  

Detection of fps tumor antigen with mono-specific anti-fps serum in tumors induced by acute transforming ALV

作　　者：王一新[1] 陈浩[2] 赵鹏[1] 李建亮[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]北京家禽育种有限公司,北京101301

出　　处：《微生物学报》2013年第3期299-305,共7页Acta Microbiologica Sinica

基　　金：国家自然科学基金面上项目(31172330)~~

摘　　要：【目的】制备鼠抗fps单因子血清,检测急性致瘤性ALV诱发肉瘤组织中的fps肿瘤抗原。【方法】以J亚群相关禽白血病/肉瘤病毒Fu-J株RNA为模板,分两段扩增v-fps肿瘤基因,利用大肠杆菌表达系统表达这两段蛋白,纯化后将两种蛋白同时免疫小鼠,获得抗血清,间接免疫荧光试验检测肉瘤组织滤过液感染的CEF和肿瘤细胞中的fps抗原,免疫组织化学方法检测肿瘤组织中的fps抗原。【结果】该单因子血清具有较好特异性,不与经典ALV-J发生交叉反应。被感染的CEF细胞、肿瘤细胞及纤维肉瘤组织切片均显示阳性。【结论】该实验制备了鼠抗fps单因子血清,建立了fps肿瘤抗原的检测方法,为进一步研究该病毒的生物学特性及其致瘤机制奠定了基础。[ Objective] To prepare anti-fps mono-specific serum, and detect the fps antigen in tumors induced by acute transforming avian leukosis/sarcoma virus containing v-fps oncogene. [ Methods] Two part of v-fps gene was amplified by RT-PCR using the Fu-J viral RNA as the template. Mono-specific serum was prepared by immuning Kunming white mouse with both two recombinant infusion proteins expressed by the prokaryotic expression system. Indirect immunofluorescent assay was used to detect fps antigen in tumor tissue suspension ceils and CEF infected by sarcoma supernatant. Immunohistochemical method was used to detect fps antigen in tumor tissue. [ Results] The mouse mono-specific serum was specific as it had no cross reaction with classical ALV-J strains. The result reveals that the tumor tissue suspension cells, the CEF infected by sarcoma supernatant, and the slice immunohistochemistry of the sarcoma showed positive results. [ Conclusion] The anti-fps mono-specific serum was prepared, and the detection method was established, which laid the foundation for the study of viral biological characteristics and mechanism of tumourgenesis of acute transforming avian leukosis/sarcoma virus containing v-fps oncogene.

关 键 词：J亚群相关禽白血病/肉瘤病毒 v-fps基因 单因子血清 间接免疫荧光试验 免疫组织化学试验 

分 类 号：S852.65[农业科学—基础兽医学]