糖皮质激素性骨质疏松模型兔的建立与3．0T磁共振波谱评价  

Establishment and evaluation with MR spectroscopy in glucocorticoid-induced osteoporosis in rabbits

作　　者：李冠武[1] 常时新[1] 范敬争[1] 史晓[2] 陈笛[1] 祝乐群[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院医学影像科,上海200437 [2]上海中医药大学附属岳阳中西医结合医院老年科,上海200437

出　　处：《实用放射学杂志》2013年第2期295-298,共4页Journal of Practical Radiology

基　　金：上海市教委基金资助项目（2010JW53）;国家自然科学基金项目（81202809）.

摘　　要：目的3．0T磁共振波谱（MRs）评估糖皮质激素性骨质疏松（GIOP）模型兔的可行性。方法19只20周龄雌性新西兰大白兔随机分为对照组（n=9）及肌注甲泼尼龙琥珀酸钠组（MPS组，n=10）。分别在基线、4周、8周行股骨近端及L3～L4骨密度扫描及MRS扫描获取骨髓脂肪分数（FF）。结果第4周时，MPS组FF（42．4％士6．3％）较对照组（32．5％土6．5％）显著增高（P=0．003），而组间骨密度无统计学差异。第8周时，MPS组股骨近端及L3-L4骨密度都较对照组明显降低（P均〈0．001）。第4、8周时MPS组FF较基线时分别增高36．3％、60．5％，而股骨近端与L3～L4骨密度分别较基线时降低4．5％、13．3％与6．5％、14．4％。结论GIOP模型兔可在8周内建立，MRs是该模型的有效评估方法之一。Objective To clarify the feasibility of evaluating the glucocorticoid-induced osteoporosis（GIOP） in rabbits with MR spectroscopy. Methods Nineteen female New Zealand white rabbits （20-week-old） were randomly assigned to two groups： MPS group （n=10） received subcutaneous injections of MPS for the first 4 weeks at a dose of 1.5 mg/kg everyday, and then at a dose of 0.35 mg/kg （three times per week） for another 4 weeks. The control group （n= 9） received subcutaneous injections of normal saline according to the same schedule. The animals were subjected to the same MR protocol and dual energy X-ray absorptiometry scanning at 4 and 8 weeks after baseline imaging. Results There was a significant increase of bone marrow fat fraction （FF） in MPS group （ 42.4 % ± 6.3% ） compared with control group （ 32.5%± 6.5 % ） （ P = 0. 003 ）, but no significant differences in both proximal femur BMD and L3-L4 bone mineral density （BMD） were detected at week 4. At week 8, both proximal femur BMD and L3-L4 BMD of MPS group were significantly lower （P〈0. 001 for all） than those of control group. In comparison with baseline, FF values in MPS group increased by 36.3 % and 60.5 % at 4, 8 weeks respectively, while proximal femur BMD and L3-- L4 BMD decreased by 4.5 and 13.3%, 6.5 % and 14.4 %. Conclusion GIOP in rabbits can be induced successfully in eight weeks. MRS may be valuable in the early, non-invasive evaluation of a rabbit osteoporosis model.

关 键 词：糖皮质激素性骨质疏松 模型 兔 磁共振波谱 骨密度 

分 类 号：R681[医药卫生—骨科学] R445.2[医药卫生—外科学]