鸡胚和雏鸡接种ALV-J相关急性纤维肉瘤浸出液的致病性比较  

Comparison of the Pathogenicity of ALV-J Related Acute Fibrosarcoma Extract Inoculated on Embryos and Chicks

作　　者：李德庆[1] 赵鹏[1] 王鑫[1,2] 汪晓飞[1] 崔治中[1] 

机构地区：[1]山东农业大学动物医学院,泰安271018 [2]中国农业科学院家禽研究所,扬州225125

出　　处：《畜牧兽医学报》2013年第2期250-255,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金面上项目(#31172330);国家公益性行业(农业)科研专项经费项目(#20123055)

摘　　要：为了比较鸡胚接种ALV-J相关急性纤维肉瘤浸出液对胚体及雏鸡的致病性,将含ALV-J相关病毒的肉瘤浸出液分别经5日龄胚卵黄囊、11日龄胚绒毛尿囊膜、1日龄雏鸡腹腔接种,比较不同接种方式对SPF鸡胚及雏鸡的致病性。结果表明,5日龄卵黄囊接种的鸡胚在18～22日龄死胚率为14/30,肿瘤发生率为8/14;11日龄绒毛尿囊膜接种的鸡胚在18～22日龄引起鸡胚死亡率为17/30,肿瘤发生率为6/17。对雏鸡的致病性比较表明,绒毛尿囊膜接种的13只出壳雏鸡全部死亡,有11只出现肿瘤。结果提示,绒毛尿囊膜接种的致病性不仅高于卵黄囊接种,也高于1日龄雏鸡接种。绒毛尿囊膜接种不仅肿瘤发生率高,且发生得更早、更快,可作为这种急性纤维肉瘤进一步作人工造病的实验模型。This experiment was conducted to compare the pathogenicity of ALV-J related acute fibrosarcoma extract inoculated on embryos and chicks.Acute fibrosarcoma extract was inoculated on yolk sac of 5-day-old-embryos,chorioallantoic membrane of 11-day-old-embryos and intraperitoneal inoculation of 1-day-old SPF chicks to compare pathogenicity of different inoculation methods.The results showed that the mortality of yolk sac inoculation was 14/30 at the age of 18-22 days,the incidence of visceral sarcoma was 8/14.The mortality of Chorioallantoic membrane inoculation was 17/30 at the age of 18-22 days,the incidence of visceral sarcoma was 6/17.As to the pathogenicity of the chicks,13 hatched chicks from the chorioallantoic membrane inoculation group were all died,11 of them developed visceral sarcoma.These results indicated that the pathogenicity of chorioallantoic membrane inoculation is higher than those inoculated on yolk sac of 5-day-old-embryos and those intraperitoneal inoculation of 1-day-old SPF chicks.Chorioallantoic membrane inoculation not only caused high incidence of visceral sarcoma,but also the sarcoma occurs earlier and faster,so it can be used as further acute fibrosarcoma model of artificial disease.

关 键 词：鸡胚接种 雏鸡 ALV-J(J亚群禽白血病病毒) 急性纤维肉瘤 

分 类 号：S852.659.3[农业科学—基础兽医学]