表达鸡传染性支气管炎病毒S1蛋白的重组假型杆状病毒对SPF鸡的免疫原性分析  

Immunogenicity of a Recombinant Pseudotype Baculovirus Expressing the Sl Protein of Infectious Bronchitis Virus in Specific Pathogen Free(SPF) Chickens

作　　者：樊惠英[1] 罗琼[1] 陈筱薇[1] 叶昱[1] 辛朝安[1] 廖明[1] 

机构地区：[1]华南农业大学兽医学院农业部兽用疫苗创制重点实验室,广州510642

出　　处：《畜牧兽医学报》2013年第2期256-261,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学青年基金(30800826);农业微生物学重点实验室开发课题(20090010);华南农业大学校长基金(5500-k08240);973计划项目(2011CB504701);863项目(2011AA10A209)

摘　　要：本研究旨在对本实验室所构建的表达鸡传染性支气管炎病毒(IBV)S1蛋白的重组假型杆状病毒(Ac-V-S1)的免疫保护效果进行探讨。以2×109 pfu剂量的重组假型杆状病毒(Ac-V-S1)腿部肌肉注射7日龄SPF雏鸡,21日龄加强免疫1次。血清抗体检测表明,灭活疫苗组诱导的IBV特异性ELISA抗体水平最高,极显著高于其它试验组(P<0.01),其次是Prime-boost组(先免疫Ac-V-S1后免疫poly156S1亚单位疫苗)和Ac-V-S1组;但在细胞免疫水平方面,Ac-V-S1免疫组表现出较强的优势,免疫应答反应极显著的高于其它免疫组(P<0.01),然后是Prime-boost组和杆状病毒野毒对照组。二免后2周用IBV M41强毒进行攻毒保护试验。结果表明,Ac-V-S1、Ac-V-EGFP、poly156S1亚单位疫苗组的保护率分别为55%(6/11)、27%(3/11)、37%(4/11),而采用Prime-boost免疫组的保护率为64%(7/11),略低于免疫保护率为73%的常规灭活疫苗(8/11)。结果显示:重组杆状病毒Ac-V-S1具有较好的免疫效果,而且在细胞免疫方面具有较强优势,可以弥补重组杆状病毒在体液免疫水平的不足,而Prime-boost免疫策略能更进一步提高重组杆状病毒免疫效果,为将重组杆状病毒发展为经济有效的IBV基因工程疫苗奠定基础。This experiment was conducted to study the immune protection effect of a Recombinant Pseudotype Baculovirus expressing the Sl protein of infectious bronchitis virus.Sixty-six 7-day-old SPF chickens were randomly divided into 6 groups,and were inoculated with Ac-V-EGFP（2×109 pfu）,Ac-V-S1,poly156S1 subunit vaccine,commercialized inactivated vaccine（M41） and PBS（control） respectively,boosted immunization was conducted at day 21.The results showed that,in the humoral immune,the inactivated vaccine group induced the highest level of IBV-specific ELISA antibody,significantly higher than other groups（P0.01）,in turn were prime-boost group and the Ac-V-S1 group;in cellular immunity,the Ac-V-S1 immunized group showed significantly higher than other groups（P0.01）,in turn were prime-boost group and the wild-type baculovirus group.Two weeks after the second immunization,the chickens were challenged with IBV M41 strain.The post-challenge results showed that the protective rate of Ac-V-S1,Ac-V-EGFP,and poly156S1 subunit vaccine were 55%（6/11）,27%（3/11）,37%（4/11）,respectively,and the protective rate of the prime-boost immunity group was 64%（7/11）,a little lower than the regular inactivated vaccine group 73%（8/11）.Results demonstrated that the recombinant baculovirus Ac-V-S1 showed better effects on cellular immunity,which can make up for deficiencies in humoral immunity,and the prime-boost immunization strategy can further enhance the immune effect of recombinant baculovirus.This study laid the foundation of the research of recombinant baculovirus as genetic engineering vaccine of IBV.

关 键 词：传染性支气管炎病毒 重组杆状病毒 S1基因 免疫保护 

分 类 号：S852.659.6[农业科学—基础兽医学]