ICR胎鼠原代神经元细胞培养及鉴定  

Culture and identification of primary neurons isolated from embryonic ICR mice

作　　者：陈雪松[1,2] 马姬[1] 薛燕[1] 曾凡一[1,2] 

机构地区：[1]上海交通大学医学院医学科学研究院发育生物学研究室,上海200025 [2]上海市儿童医院卫生部医学胚胎分子生物学重点实验室暨上海市胚胎与生殖工程重点实验室,上海200040

出　　处：《上海交通大学学报（医学版）》2013年第2期249-252,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81125003;30900259);国家高技术研究发展计划项目(2011AA020116);上海市科委优秀学术带头人项目(12XD1406500);上海市科委项目(10140900200);国家重点基础研究发展计划项目(2010CB945200)~~

摘　　要：目的建立较理想的胎鼠神经元细胞体外原代培养方法。方法取13.5 d ICR胎鼠的大脑皮层,经剪碎消化得到单细胞悬液进行培养,观察细胞形态并作PCR及Western blotting等进行神经元相关的基因及蛋白Tuj1和Map2的鉴定。结果细胞生长状态良好,细胞胞体明显,周围有明亮光晕,细胞突触交错形成网络样,通过PCR及Western blotting验证证明所分离培养的细胞是神经元细胞。结论本培养方法简单易行,可获得典型和纯度较高的ICR胎鼠大脑皮层神经元细胞。Objective To establish a favorable primary culture technique for neurons isolated from embryonic ICR mouse cortical tissues. Methods The cortex of embryonie ICR mice aged 13.5 d was isolated, mechanically dissected and digested, and was proeeeded to culture. The morphology of neurons was observed, and PCR and Western blotting were applied to identify the expression of Tujl and Map2 gene and protein in neurons. Results Cells grew well, with distinct cell body and surrounding bright halation, and there was typical nerve fiber network of synapses. The isolated and cultured ceils were confirmed as neurons by PCR and Western blotting. Conclusion This technique is an easy and practical tool for the primary culture of embryonic ICR mouse cortical neurons with high purity.

关 键 词：神经元 原代培养 ICR胎鼠 疾病模型 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]