大鼠脊髓压迫性损伤后脱髓鞘病变及MBP、Id2的表达变化  

Axonal demyelination and alteration of MBP and Id2 expression after compressed spinal cord injury

作　　者：黄思琴[1] 漆伟[2] 孙善全[2] 汪克建[2] 卓飞[2] 蒋锦[2] 

机构地区：[1]重庆医科大学中医药学院,重庆400016 [2]重庆医科大学神经科学研究中心,重庆400016

出　　处：《第三军医大学学报》2013年第5期381-384,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30270437);重庆市自然科学基金(CSTC2012jjA10020);重庆市教委科学技术研究项目(KJ120312);重庆市渝中区科技计划项目(20110316);重庆市卫生局中医药科技项目(2012-2-138)~~

摘　　要：目的分析脊髓压迫性损伤(compressed spinal cord injury,CSCI)后脱髓鞘病变与髓鞘碱性蛋白(myelinbasic protein,MBP)、DNA结合抑制物2(inhibitor of DNA binding2,Id2)的表达变化之间的关系,以探讨CSCI脱髓鞘病变机制。方法采用自行设计的方法制作SD大鼠CSCI模型,通过锇酸染色检测CSCI后1、3、7 d有髓神经纤维变化;运用免疫荧光双标和免疫印迹(Western blot)检测MBP及Id2的表达变化。结果 CSCI后出现脱髓鞘病变,并随着压迫时间延长,髓鞘逐渐发生水肿、变性、崩解;脊髓损伤后MBP表达下调,其表达趋势与脱髓鞘溃变的严重程度一致;CSCI后,Id2广泛分布于白质,随着压迫时间延长,其表达逐渐上调。结论 Id2表达上调,并负向调控MBP基因启动子的活性,使MBP的表达下降,是CSCI后神经纤维脱髓鞘病变的机制之一。Objective To investigate the relationship between axonal demyelination after compressed spinal cord injury （CSCI） and expression of myelin basic protein （MBP） and inhibitor of DNA binding 2 （Id2）, and to explore the mechanism of axonal demyelination after CSCI. Methods The CSCI model was established with a self-made device. The changes of myelinated nerve fibers in white matter were determined by osmic acid staining at 1, 3 and 7 d following CSCI. MBP and Id2 expression levels were observed by double-labeling immunofluorescence and Western blotting. Results Axonal demyelination occurred after CSCI and myelin sheath became swelling, degenerative and breakdown with time extending. The expression of MBP was down-regulated after CSCI, which was consistent with the degree of demyelination. Id2 distributed widely in white matter, and its expression increased along with time extending after CSCI. Conclusion MBP and Id2 are associated with axonal demyelination, and may contribute to axonal demyelination after CSCI.

关 键 词：脊髓压迫性损伤 脱髓鞘病变 髓鞘碱性蛋白 DNA结合抑制物2 

分 类 号：R341[医药卫生—基础医学] R363.2