IRE1α促进人牙周膜成纤维细胞增殖  

Effect of IRE1α on proliferation of human periodontal ligament fibroblasts

作　　者：彭志庆[1] 李苹苹[2] 初颜兵[3] 王燕[4] 

机构地区：[1]重庆医科大学附属永川医院口腔科,重庆402160 [2]重庆医科大学附属口腔医院上清寺牙体牙髓科,重庆400015 [3]第三军医大学西南医院口腔科,重庆400038 [4]重庆医科大学附属口腔医院儿童牙病中心及口腔预防科,重庆401147

出　　处：《第三军医大学学报》2013年第5期435-437,共3页Journal of Third Military Medical University

摘　　要：目的研究内质网跨膜蛋白IRE1α对人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hPDLFs)增殖的影响。方法将成功构建的人IRE1α基因重组质粒染入hPDLFs细胞,采用免疫印迹法检测IRE1α重组基因在真核细胞内的表达情况,XTT法和流式细胞仪(FCM)检测转染后hPDLFs细胞增殖和细胞周期变化。结果酶切及测序结果证实IRE1α重组质粒构建成功;免疫印迹分析结果证实,IRE1α重组质粒能在hPDLFs细胞内正确表达;在内质网应激(ERS)状态下,与衣霉素(tunicamycin,TM)对照组相比,XTT检测结果显示:IRE1α实验组hPDLFs细胞增殖率明显增高(P<0.01);FCM结果分析显示:IRE1α实验组hPDLFs细胞S期比例增加而G1期减少(P<0.05)。结论在ERS状态下,IRE1α促进hPDLFs细胞增殖,促进hPDLFs细胞从G1期进入S期。Objective To study the effect of IRE1α on the proliferation of human periodontal ligament fibroblasts （hPDLFs）. Methods The IRE1α full-length eukaryotic expression vectors were constructed and transiently transferred into hPDLFs, and the expression of IRE1α was identified by Western blot analysis. The proliferation of hPDLFs was analyzed by XTT assay, and the changes of cell cycle were detected by flow cytometry （FCM）. Results Restriction enzyme digestion and gene sequencing identified that the IRE1α recombinant plasmid was successfully constructed, and the correct expression of IRE1α in hPDLFs was detected by Western blotting. Compared with the control group （tunicamycin）, the XTT results showed that the cell proliferation rate of the IRE1α group increased significantly （P〈0.01）, and the FCM results showed that the cell proportion increased at S phase and decreased at G1 phase in the IRE1α group （P〈0.05）. Conclusion IRE1α can improve hPDLFs proliferation, and promote hPDLFs from G1 phase into S phase.

关 键 词：IRE1α 牙周膜成纤维细胞 细胞增殖 细胞周期 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R341[医药卫生—基础医学]