SPF和正常鼠下呼吸道菌群多样性研究  

Study on low respiratory tract microbiota diversity of SPF and normal mouse

作　　者：于文凯[1] 刘越坚[2] 唐立[1] 袁晓鹏[1] 徐星澈[1] 谭丽莎[1] 李坤[1] 戴凤翠[1] 刘银辉[1] 李明[1] 李华军[1] 郭燕杰[1] 袁杰利[1] 文姝[1] 

机构地区：[1]大连医科大学微生态学教研室,辽宁大连116044 [2]大连医科大学附属第一医院中心实验室,辽宁大连116011

出　　处：《中国微生态学杂志》2013年第2期132-134,共3页Chinese Journal of Microecology

基　　金：国家自然科学基金资助(81150014)

摘　　要：目的探讨SPF和正常鼠下呼吸道菌群多样性区别,为研究洁净环境下呼吸道菌群对免疫耐受形成的影响提供简便的动物模型。方法采用飞行质谱和DGGE的方法检测正常和SPF BALB/c小鼠及Wistar大鼠呼吸道支气管肺泡灌洗液中菌群多样性的区别。结果 SPF BALB/c小鼠下呼吸道菌群丰度小于普通小鼠,下呼吸道菌群丰度小于消化道。SPF Wistar大鼠下呼吸道菌群丰度小于普通大鼠.结论 SPF环境造成鼠下呼吸道菌群丰度减小。Objective To analyze the low respiratory tract microbiota diversity difference between SPF and normal mouse,provide a convenient animal model for the study of the effect of low respiratory tract microbiota on immune tolerance in clean environment.Methods Flight mass spectrometry and DGGE method were used to detect the microbiota diversity difference between normal and SPF mouse respiratory Bronchoalveolar lavage.Results The low respiratory tract microbiota diversity of SPF BALB/c mice was less than the normal mice,and the respiratory tract microbiota diversity less than the digestive tract.SPF Wistar rat low respiratory tract microbiota diversity was less than ordinary rats.Conclusion The low mouse respiratory tract microbiota diversity decreases in SPF environment.

关 键 词：下呼吸道菌群 多样性 SPF鼠 

分 类 号：R378[医药卫生—病原生物学]