和厚朴酚对根管内白色念珠菌生物膜作用的体外研究  

Effect of honokiol on Candida albicans biofilms in root canals in vitro

作　　者：田玉珠[1] 王健平[1] 杨景云[2] 张慧明[2] 杨玉娟[1] 

机构地区：[1]佳木斯大学附属口腔医院,黑龙江佳木斯154003 [2]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《中国微生态学杂志》2013年第2期154-156,共3页Chinese Journal of Microecology

基　　金：黑龙江省卫生厅课题(2009-364)

摘　　要：目的通过观察和厚朴酚对体外白色念珠菌生物膜形成中的作用,探讨其在口腔微生态中变化的意义。方法采用XTT减低法评价和厚朴酚对白色念珠菌的生物膜及黏附性的影响;利用激光共聚焦扫描显微镜和死菌活菌荧光染色技术相结合,对常态及药物作用下白色念珠菌生物膜厚度及活性进行观察。结果与阴性对照组相比,15.63、31.25及62.5μg/mL的和厚朴酚对白色念珠菌的早期黏附及菌丝生长有抑制作用;2 000～15.63μg/mL的和厚朴酚对白色念珠菌生物膜的抑菌率分别为90.13%～24.21%;24 h时常态生物膜结构中大部分是活菌,有少量死菌存在,生物膜厚度为(75.15±6.57)μm。Image-Pro Plus 6.0软件分析显示,62.5μg/mL的和厚朴酚对24 h生物膜作用后活菌百分比为(31.4±0.09)%,生物膜厚度为(33.14±6.66)μm,与阴性对照组相比,差异有统计学意义(P<0.05)。与制霉菌素相比,其抑菌活性相对较弱,但对生物膜厚度影响强于制霉菌素。结论和厚朴酚对体外白色念珠菌生物膜有抑制作用。Objective To investigate the significance of honokiol in the oral microecology changes by observing its effect on Candida albicans biofilms in vitro.Methods XTT reduction assay was applied to evaluate the effect of honokiol on C.albicans biofilms and its adhesion;Confocal laser scanning microscopy combining with vital fluorescent staining technique was used to observe the thickness and activity of C.albicans biofilms with and without the drug action.Results 15.63 μg/mL,31.25 μg/mL and 62.5 μg/mL of honokiol had inhibited the early adhesion and mycelial growth of C.albicans;the inhibition rates of 2000 μg/mL to 15.63 μg/mL honokiol against C.albicans biofilms were 90.13% to 24.21%;Viable bacteria was dominant at 24 h in the untreated C.albicans biofilms,and the thickness of the C.albicans biofilms was（75.15±6.57） μm;The quantitative data from Image-Pro Plus 6.0 software showed that under the inhibition of 62.5 μg/mL honokiol at 24 h,the viable bacteria percentage was（31.4±0.09）% and the biofilm thickness was（33.14±6.66） μm;compared with the negative control group,the difference was statistically significant（P〈0.05）.The antibacterial activity of honokiol was relatively weaker than that of nystatin,but its influence on biofilm thickness was stronger.Conclusion Honokiol has inhibition against C.albicans biofilms in vitro.

关 键 词：白色念珠菌 生物膜 和厚朴酚 激光共聚焦扫描显微镜 

分 类 号：R379.4[医药卫生—病原生物学]