应用PCR-DGGE分析肉鸡呼吸道中细菌多样性  

Bacteria diversity in respiratory passage of chiken estimated by PCR-DGGE

作　　者：汪孟娟[1] 曹银生[1] 胡艳霞[1] 王京京[1] 辛国芹[1] 徐海燕[1] 

机构地区：[1]山东宝来利来生物工程股份有限公司

出　　处：《中国饲料》2013年第4期26-28,共3页China Feed

摘　　要：从不同养殖时期的肉鸡呼吸道中提取微生物基因组总DNA,以细菌16S rDNA基因通用引物341F/534R进行V3高变异区域PCR扩增,长约200 bp的PCR产物经变性梯度凝胶电泳(DGGE)分离,获得微生物群落的特征DNA指纹图谱。通过DGGE图谱的半定量分析以及对条带的测序鉴定,发现样品的优势菌群明显。结果表明,PCR-DGGE是研究动物呼吸道微生物多样性的可行方法。The microbial genomic DNA was extracted from respiratory passage of chicken in different breeding period .The 16S rDNA genes （V3 region）were amplified by PCR with the universal primers （341F/534R）,the products with a length of about 200 bp were separated by denaturing gradient gel electrophoresis （DGGE）, DNA fingerprinting of the mi- crofiora was obtained.The dominant microflora in samples were easily found by semi-quantitative analysis of the DGGE profiles and appraisal of sequencing of the strip.The results showed that PCR-DGGE is a feasible method to study the mi- crobial diversity of animal respiratory passage.

关 键 词：PCR—DGGE 肉鸡 呼吸道 细菌多样性 

分 类 号：S811.6[农业科学—畜牧学]