高效阴离子交换-脉冲安培检测壳寡糖在体外非翻转肠囊模型中的吸收  

Detection of Chitooligosaccharides Absorption in Non-everted Rat Intestinal Sacs Model in vitro with High Performance Anionexchange Chromatography with Pulsed Amperometric Detection

作　　者：魏鹏[1,2] 赵小明[1] 许青松[1] 谭成玉[3] 白雪芳[1] 杜昱光[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116021 [2]中国科学院研究生院,北京110864 [3]大连海洋大学,辽宁大连116023

出　　处：《西北农业学报》2013年第1期6-11,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31072065);"十二五"国家科技支撑课题(2011BAD26B02-3)

摘　　要：建立高效阴离子交换色谱结合脉冲安培检测器(HPAEC-PAD)检测非翻转肠囊中壳寡糖吸收的方法。结果表明,以RCX-10阴离子交换柱为分离柱,通过NaOH溶液的梯度淋洗,壳一糖～壳六糖的检出限为0.8～4.0mg/L(进样体积100μL,峰面积定量),该方法的相对标准偏差为1.3%～6.6%。体外非翻转大鼠肠囊模型中,壳二糖、壳三糖以及壳四糖在十二指肠、空肠以及回肠中均有良好的吸收(Papp>1×10-5 cm/s),在结肠中仅能部分吸收(Papp<3×10-6 cm/s)。壳寡糖吸收速率随聚合度的升高而降低,壳寡糖吸收符合一级吸收动力学特征,推测壳寡糖在体内的吸收为被动过程。A method for determination of chitooligosaccharides（COS） in non-everted intestinal sacs model by high performance anion-exchange chromatography with pulsed amperometric detection（HPAEC-PAD） was established.The chromatographic separation was established on a Hamilton RCX-10 anion exchange column using NaOH gradient elution.Detection limits of 0.8-4.0 mg/L（100 μL injection volume） were achieved under optimized conditions.RSD of the peak area for COS was 1.3%-6.6%.The results demonstrated chitobiose,chitotriose and chitotetraose could be well absorbed in duodenum,jejunum and ileum with high apparent permeability coefficients（Papp1×10-5cm/s）,and the absorption of COS in colon was low（Papp3×10-6cm/s） in non-everted intestinal sacs in vitro.It was also proven that as polymerization degree of COS increased,the absorption rate decreased.The mechanism of COS absorption was in a passive absorption manner and followed the first-order absorption kinetics.

关 键 词：壳寡糖 吸收 非翻转肠囊法 高效阴离子交换色谱 脉冲安培检测 

分 类 号：R965.1[医药卫生—药理学]