Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize  

作　　者：YU Jiang-hua ZHAO Yan-xin QIN Ya-ting YUE Bing ZHENG Yong-lian XIAO Hai-lin 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University

出　　处：《Journal of Integrative Agriculture》2013年第2期229-238,共10页农业科学学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(31171565)

摘　　要：MicroRNAs （miRNAs） are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value〈0.01 and 0.01〈P-value〈0.05, respectively. Four miRNAs showing 〉1.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes.MicroRNAs （miRNAs） are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value〈0.01 and 0.01〈P-value〈0.05, respectively. Four miRNAs showing 〉1.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes.

关 键 词：CMS-S MICRORNAS real-time PCR Solexa sequencing target gene MAIZE 

分 类 号：S513[农业科学—作物学]