六肽对兔血小板聚集活性的影响  

Influence of Hexapeptide on Rabbit Platelet Aggregation Activity

作　　者：龙丽辉[1,2] 郑艳侠[1] 陈琳[1] 徐利[1] 曹永孝[2] 刘静[2] 韩燕[2] 

机构地区：[1]西安医学院附属医院,西安710077 [2]西安交通大学医学院,西安710061

出　　处：《中国现代应用药学》2013年第2期118-121,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：陕西省教育厅项目(11JK0722);西安医学院附属医院项目(XYFY11-02)

摘　　要：目的研究六肽对兔血小板聚集活性的影响。方法采集健康家兔颈动脉血,以枸橼酸钠抗凝,用比浊法测其血小板在不同诱导剂诱导下聚集率。结果 1×10 5mol.L 1六肽,对兔ADP、花生四烯酸(AA)和凝血酶诱导的血小板聚集的抑制率分别为(66.22±1.40)%,(67.94±2.32)%和(58.18±4.67)%。六肽抑制兔ADP、AA和凝血酶诱导的血小板聚集的IC50分别为3.24×10 6mol.L 1,1.32×10 6mol.L 1和7.24×10 6mol.L 1。结论六肽在体外具有抑制兔血小板聚集的作用。OBJECTIVE To investigate the effect of hexapeptide on platelet aggregation activity in rabbit. METHODS The blood of rabbit carotid artery was taken, and then, the anticoagulation blood was obtained by adding sodium citrate into the whole blood. The platelet aggregation rates induced by different inducers were determined by turbidimetry. RESULTS The aggregation inhibition rates of 1×10^5mol.L -1 hexapeptide induced by ADP, arachidonic acid and thrombin in rabbit, were （66.22±1.40）%, （67.94±2.32）% and （58.18±4.67）%, respectively. The IC50 of aggregation inhibition rates of hexapeptide in rabbit induced by ADP, arachidonic acid and thrombin, were 3.24± 10-6 mol.L-l, 1.32×10^6mol.L -1 and 7.24×10^6mol.L -1, respectively. CONCLUSION Hexapeptide has the effect ofanti-platelet aggregation in rabbit in vitro.

关 键 词：六肽 血小板聚集 二磷酸腺苷 花生四烯酸 凝血酶 

分 类 号：R965[医药卫生—药理学]