牛流行热病毒山东地方株的分离与鉴定  

Isolation and Identification of Bovine Ephemeral Fever Virus Strain in Shandong Province

作　　者：王洪梅[1] 侯佩莉[1] 杨宏军[1] 宋玲玲[1] 何洪彬[1] 

机构地区：[1]山东省农业科学院奶牛研究中心,山东济南250131

出　　处：《山东农业科学》2013年第2期31-33,37,共4页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2010CM012);现代农业(奶牛)产业技术体系项目;泰山学者海外特聘专家项目;国家自然科学基金项目(31072160);济南市高校院所主创新计划项目(201004027;201202059;201102034);山东省科技攻关课题(2009GG20002032);山东省青年自然科学基金项目(ZR2010ZR029)

摘　　要：利用BHK-21细胞,接种山东济南地区疑似牛流行热病牛高热期的抗凝血,经盲传3代,分离到1株病毒,经RT-PCR鉴定为牛流行热病毒,命名为BEFV-SD。理化特性试验表明:BEFV-SD对热敏感,56℃10 min、37℃18 h可被灭活,对乙醚、氯仿等敏感;BEFV-SD可被BEFV阳性血清中和,中和效价为1∶408;将BEFV-SD的G基因与GenBank中公布的BEFV进行同源性比较,同源性达到96.3%以上。One virus strain named as BEFV - SD was isolated from the infected cattle in Shandong Prov- ince, and it was systematically identified by a series of methods through cultivated in BHK -21 cells. In re- sult, the isolate could cause typical cytopathogenic effect in the BHK- 21 cells after infection. The BEFV - SD could be neutralized by the positive sera with bovine ephemeral fever virus at the titer of 1 ： 408. The isolate virus was sensitive to chloroform, diethyl ether and heat, and could be inactivated at 56℃ for 10 minutes or 37℃ for 18 hours. The G gene was amplified by RT -PCR from the BHK -21 cells infected with BEFV -SD virus. The homology of G gene nucleic acid sequences among the virus in GenBank was more than 96. 3%.

关 键 词：牛流行热病毒 分离 鉴定 同源性 

分 类 号：S855.3[农业科学—临床兽医学]