锌指核酸酶技术在动物转基因研究中的应用  

Application of Zinc Finger Nuclease in Animal Transgenic Research

作　　者：刘晓[1,2] 方永志 刘文浩[2] 王洪梅[2] 何洪彬[2] 

机构地区：[1]山东师范大学,山东济南250014 [2]山东省农业科学院奶牛研究中心,山东济南250131 [3]济南军区军犬训练大队,山东济南250100

出　　处：《山东农业科学》2013年第2期135-138,共4页Shandong Agricultural Sciences

基　　金：泰山学者海外特聘专家(何洪彬);国家奶牛产业技术体系建设专项经费(何洪彬);山东省科技攻关课题(2009GG20002032);国家转基因重大专项(2009ZX08007-006B;2011ZX08007-002;2011ZX08008-004);济南市高校院所自主创新计划(201004027)

摘　　要：锌指蛋白核酸酶(Zinc Finger Nuclease,ZFN)是一种人工合成酶,含有锌指蛋白和FokⅠ核酸内切酶的剪切结构域,因其能特异性识别并切割DNA序列及其可设计性而被用基因定点突变和外源基因定点整合。这项技术已应用于转基因动物研究中。本文综述了锌指核酸酶技术在转基因研究中的应用进展,并对锌指核酸酶技术的应用前景进行了展望。Zinc finger nuclease （ZFN） is a kind of artificial synthesis containing zinc finger protein and the shear structure domain of Fok I endonuclease. Because it can specifically identify and cleave DNA se- quences and easily be designed, it is used for the site - directed genetic mutations and integration of exogenous genes. This technology has been applied to the research of transgenic animals. In this paper, the research pro- gress of zinc finger nuclease technology and its application were reviewed, and its application prospect was also forecasted.

关 键 词：锌指蛋白 锌指核酸酶 基因打靶 同源重组 转基因动物 

分 类 号：Q503[生物学—生物化学]