水通道蛋白3在小鼠早期胚胎发育过程中的表达  

Expression and distribution of aquaporin 3 during early embryonic development of mouse

作　　者：农璎琦[1] 刘风华[1] 陈烨[1] 刘怡潼[1] 阮建兴[1] 

机构地区：[1]广东省妇幼保健院生殖健康与不孕症科,广州510010

出　　处：《中华围产医学杂志》2013年第2期86-89,共4页Chinese Journal of Perinatal Medicine

摘　　要：目的了解水通道蛋白3（aquaporin3，AQP3）在小鼠早期胚胎发育中的表达分布情况。方法建立昆明小鼠控制性超排卵模型，收集小鼠四细胞期、八细胞期、桑葚期胚胎及早期囊胚，采用免疫荧光显微镜和激光共聚焦显微镜对AQP3在小鼠早期胚胎中的表达模式及亚细胞定位进行检测分析。结果在小鼠早期胚胎的4个时期中均能检测到特异性AQP3荧光信号，激光共聚焦显微镜观察到AQP3的分布部位在各时期不完全相同，在四细胞期胚胎及八细胞期胚胎主要定位于卯裂球核膜，桑葚期胚胎主要定位于细胞膜，而早期囊胚主要定位于滋养层细胞膜及细胞质。结论AQP3对小鼠早期胚胎的发育可能具有潜在的调控作用。Objective To investigate the expression and distribution of aquaporin 3 （AQP3） in mouse early embryos at different stages. Methods Controlled ovarian hyperstimulation model of Kunming mouse was used to collect four-cell embryos, eight cell embryos, morula stage, and early blastocysts. Immunofluoreseence microscopy and laser confocal microscopy were used to detect expression and distribution of AQP3 channels in these stages. Results Fluorescence signal of AQP3 was found in four embryonic stages of mice. Distribution within embryo was different at different embryonic stages. AQP3 was mainly expressed on the karyotheca of blastomeres at four-cell and eight-cell stage. In morula stage, AQP3 was mainly expressed on cell membrane of each blastomere. In early blastocysts, AQP3 was predominantly expressed on the cell membrane and cytoplasm of trophoblastic cell. Conclusions AQP3 trans-membrane channel might have potential regulation function on mouse embryonic development.

关 键 词：水通道蛋白质3 胚胎发育 显微镜检查 荧光 小鼠 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]