非人灵长目动物-食蟹猴肾移植模型的建立  

The establishing of renal transplantation model in non-human primate

作　　者：张川[1] 祝青国[1] 金承洛[1] 赵亚昆[1] 仇宇[1] 李昆仑[1] 袁刚军[1] 

机构地区：[1]哈尔滨医科大学附属第二医院泌尿外科,黑龙江省哈尔滨150086

出　　处：《中国基层医药》2013年第3期326-328,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：基金项目：黑龙江省科技计划项目（LC08C16）

摘　　要：目的探索建立非人灵长目动物一食蟹猴的肾移植模型，总结模型建立中需要注意的问题。方法气管插管麻醉下，取配型良好雄性食蟹猴50只行同种异体肾移植，将供肾动脉、肾静脉分别与受体腹主动脉、下腔静脉行端侧吻合术，尿路重建采用输尿管．膀胱吻合法。术后常规抗排斥治疗及抗炎治疗。结果50只肾移植食蟹猴中1只因手术过程中麻醉意外死亡；有7只术后出现并发症，且均伴有肌酐值的突然升高，行移植。肾及血管超声检查，其中2只肾静脉血栓形成，5只出现移植肾输尿管尿漏。所有动物模型无手术感染出现，抗排斥治疗下存活良好，血肌酐及尿量正常。结论应用非人灵长目动物一食蟹猴建立肾移植模型方法可靠，但应注意手术技术的训练，术后并发症的预防。应用此模型进行肾移植免疫耐受、异种肾移植研究等可为临床试验提供更为准确可靠的依据。Objective To explore renal transplantation model in non-human primate cynomolgus monkeys. Methods 50 non-human primates＇ kidneys were transplanted into the lower part of the abdomen with end-to-side an- astomosis of renal artery to aorta and renal vein to inferior vena eava, and with end-to-end anastomosis of ureter to bladder. Results In the 50 cases, 1 case death as accident of anesthesia;7 cases with postoperative complications, and all with creatinine sudden rise, after ultrasonic examinations showed that 2 cases with renal vein thrombosis, and 5 eases appeared urinary leakage. All animal models were without surgical infections, and with normal serum ereatinine, urine output. Conclusion Non-human primate animal kidney transplantation model establishment method is reliable, but should pay attention to the the surgical technique training, complications prevention. The model is valuable for ap- plication in the research of immune tolerance, heterogeneous transplant.

关 键 词：灵长目 猕猴属 肾移植 模型 动物 

分 类 号：R699[医药卫生—泌尿科学] R-332[医药卫生—外科学]