金属卟啉白蛋白结合体模拟SOD酶性能研究  

Antioxidant activity of conjugation of metalloporphyrin combining with bovine serum albumin

作　　者：殷晓春[1,2,3] 李刚[1,2,3,4] 王荣民[1,2,3] 何玉凤[1,2,3] 熊玉兵[1,2,3] 

机构地区：[1]生态环境相关高分子材料教育部重点实验室 [2]甘肃省高分子材料重点实验室 [3]西北师范大学化学化工学院,兰州730070 [4]兰州生物制品研究所,兰州730046

出　　处：《中国科学：化学》2013年第2期171-177,共7页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(21263024;21244003);甘肃省科技支撑计划项目(1011GKCA017);教育部"长江学者和创新团队发展计划"(IRT1177);甘肃省属高校基本科研业务费专项资金项目(2010-176)资助

摘　　要：将两类水难溶性金属卟啉(MP),meso-四苯基卟啉金属配合物(MTPP,M=ZnII,CoII)和meso-四(p-羟基苯基)卟啉金属配合物(MTpHPP,M=ZnII,CoII)分别与牛血清白蛋白(BSA)结合,制得水溶性金属卟啉白蛋白结合体(MP@BSA).采用UV-vis光谱、聚丙烯酰胺凝胶电泳(PAGE)、圆二色谱进行了表征与分析,发现金属卟啉的水溶性和稳定性得到大幅度提高.采用NBT光还原法测定了MP@BSA结合体清除超氧阴离子自由基(O2·-)的能力,发现结合体MP@BSA具有清除O2·-的性能,与小分子金属卟啉相比,结合体的抗O2·-性能提高了一个数量级.MP@BSA抗氧化活性分别是VC和BSA的600倍和40倍.其中,羟基取代金属卟啉结合体表现出更强的清除O2·-的活性,CoTpHPP@BSA的EC50为1.5μmol/L,对天然Cu,Zn-SOD的模拟度为2.73%.最后提出了生物高分子结合体清除O2·-的可能机理.In this work, two kinds of water-insoluble metalloporphyrin （MP）, meso-tetraphenylporphyrin metal complexes （MTPP, M： Zn^Ⅱ, Co^Ⅱ） and meso-tetra（p-hydroxylphenyl）porphyrin metal complexes （MTpHPP, M： Znn, Con）, were combined into bovine serum albumin （BSA）, a kind of stable and soluble biopolymer. The obtained water-soluble metalloporphyrin at BSA conjugates （MP@BSA） have been characterized by UV-vis spectra, PAGE gel electrophoresis and circular dichroism spectra. It indicated that the water-solubility and stability of metalloporphyrins were significantly increased after being bound into bovine serum albumin. Their SOD-like activities for scavenging superoxide anion free radical （O2-） were determined by NBT assay and compared with natural antioxidant. The activities of MP@BSA were more decuple than that of metalloporphyrins. The ECso values of MP@BSA were 600-fold and 40-fold higher than that of Vitamin C （VC） and BSA, respectively. The hydroxyl substituted metalloporphyrin showed more active than that of MTPP, and ECso of CoTpHPP@BSA got to 1.5 μmol/L. Compared with natural Cu,Zn-SOD, the analog quantity of CoTpHPP@ BSA reached 2.73%.

关 键 词：金属卟啉 白蛋白 抗氧化 超氧阴离子自由基 模拟酶 

分 类 号：O629.8[理学—有机化学]