表没食子儿茶素没食子酸酯通过活性氧生成抑制细菌的生长  

Antimicrobial Action of Epigallocatechin-3-gallate through Reactive Oxygen Species

作　　者：申羽佳[1] 崔海燕[1] 张东芳[1] 王坤英[1] 李卫国[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2013年第1期115-118,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省教育厅自然科学基金(2010A180011);河南师范大学生物学一级学科建设基金

摘　　要：表没食子儿茶素-3-没食子酸酯(EGCG)具有显著的抗氧化等生物学作用.运用平板菌落计数法,测定了5、10、20mg/L EGCG抑制细菌生长繁殖的效应,分析了200U/mL过氧化氢酶和80mg/L N-乙酰半胱氨酸对EGCG抑菌作用的影响机制.结果显示EGCG以剂量依赖方式抑制表皮葡萄球菌(S.epidermidis)和大肠杆菌(E.coil)的生长繁殖,而过氧化氢酶和N-乙酰半胱氨酸则减弱EGCG的抑菌作用,表明EGCG抑制细菌生长的作用可能是通过生成活性氧介导的.Epigallocatechin-3-gallate （EGCG） has an important role in anti-oxidation and bactericidal action. In this study,we use the method of plate culture count to examine the antimicrobial activity of EGCG against S. epidermidis and E. coil at 5, 10, and 20 mg/L, and to analyze the influence of catalase and N-acetylcysteine（NAC） on the antimicrobial action of EGCG. The results show that EGCG inhibits growth of two bacteria in dose-dependence fashion. Both of catalase and N-acetyl- cysteine partly eliminate the antimicrobial activity of EGCG. The results suggest that the antimicrobial action of EGCG mediated by reactive oxygen species production.

关 键 词：表没食子儿茶素-3-没食子酸酯 抑菌作用 活性氧 过氧化氢酶 N-乙酰半胱氨酸 

分 类 号：Q936[生物学—微生物学] Q939.93