间歇缺氧大鼠NF—kBp65以及MCP-1的表达  

Express of NF-kB65 and MCP-1 in intermittent hypoxia rats

作　　者：徐晓梅[1] 樊荣[1] 黄晓颖[1] 林乾顶[1] 王良兴[1] 

机构地区：[1]温州医学院附属第一医院呼吸内科,325000

出　　处：《浙江医学》2013年第1期14-15,19,共3页Zhejiang Medical Journal

基　　金：温州市科技局资助项目（Y20090125）

摘　　要：目的探讨间歇缺氧对大鼠血清以及心肌中NF—kBp65以及MCP-1含量的影响。方法清洁SD大鼠30只，随机分成3组（每组10只）：正常对照组、间歇缺氧组、持续缺氧组，普通饲料饲养。正常对照组：常压、常氧下饲养；间歇低氧组：每90s-循环，氧舱内低氧浓度维持在6％～8％，持续10s以上；持续低氧组：氧舱内氧浓度维持在8％～11％。每天早上9：00动物入舱，至下午5：00试验结束，每天持续8h，连续35d。然后经右颈动脉抽血，处死大鼠分离其心脏，采用酶联免疫吸附法（ELISA法）测定血清中NF—kBp65以及MCP～1含量，采用免疫印迹法测定大鼠心肌中NF—kBp65以及MCP-1的含量。结果与正常对照组相比，在间歇缺氧组以及持续缺氧组中大鼠血清以及心肌中NF—kBp65以及MCP-1的含量明显增加。结论间歇缺氧可导致NF—kB的激活，从而导致大鼠血清以及心肌中NF—kBp65以及MCP-1的表达增加。Objective To investigate the expression of NF-kBp65 and MCP-1 in serum and heart muscle in intermittent hypoxia rats. Methods There are thirty SD rats were randomly assigned into three groups： control group, intermittent hypoxia group and continuing hypoxia group, which were exposed to air, intermittent hypoxia and continuing hypoxia respectively 8h/d for 35d.Then the blood and heart samples were collected, and the NF-kBp65 and MCP-1 levels in serum and in heart tissue were measured with ELISA and Western blot respectively. Results Compared to the control group the contents of NF-kBp65 and MCP-1 in serum and heart were increased both in intermittent hypoxia group and continuing hypoxia group. Conclusion NF-kBp65 and MCP-1 in heart and blood of intermittent hypoxia rats, which may be associated with the activation of NF-kB by hypoxia.

关 键 词：缺氧 心肌 NF—kBp65 MCP-1大鼠 

分 类 号：R543.502[医药卫生—心血管疾病]