发酵支原体和梨支原体致死性感染小鼠胸腺细胞Fas／FasL表达与多器官损伤  

Expression of Fas/FasL in thymocytes and its relation with multi-organ damage in mice lethally infected by Mycoplasmafermentansand Mycoplasma pirum

作　　者：温秀妹[1] 沈丹丹[2] 周丽萍[2] 

机构地区：[1]温州医学院附属第一医院实验诊断中心,325000 [2]温州医学院检验医学院生命科学学院

出　　处：《浙江医学》2013年第2期99-101,F0003,共4页Zhejiang Medical Journal

基　　金：温州市科技计划项目（Y20110107）

摘　　要：目的探讨发酵支原体（Mf）和梨支原体（Mpi）感染小鼠胸腺细胞Fas／FasL表达与多器官损伤相关性。了只清洁级ICR小鼠，分实验组24只（免疫抑制Mf、Mpi感染组）及对照组31只（非免疫抑制Mf感染和NS组）。感染组分别月03mlMf和Mpi标准菌株（6×10s8-9／ml），NS组注入相同量09％氯化钠注射液。计算各组小鼠死亡率，取其血清进行支原体再疫组化法分析小鼠胸腺细胞Fas／FasL的表达，电镜观察死亡小鼠心、肺、肝和肾的超微结构变化。结果实验组小鼠在感；后100％死亡（24／24），与非免疫抑制Mf感染对照组比较有统计学差异（P〈0．01）。胸腺细胞Fas／FasL高表达与心、肝、肺和肾官损伤相一致。结论Mf或Mpi感染小鼠胸腺细胞Fas／FasL高表达与心、肺、肝和肾多器官损伤相关。Objective TO investigate expression of Fas/FasL in thymocytes and its relation with multiple organ damage in mice lethally infected by Mycoplasma fermentans （Mf） and Mycoplasma pirum （Mpi）. Methods 55 ICR mice were randomly di- vided into experimental group （immunosuppression＋Mf and Mpi infection, n=24） and control groups （non-immunosuppres- sion＋Mf infection, and normal group, .=31）. Mice in experiment group were injected intraperitoneally with 0.3ml （6 x 108-9/ml） Mf or Mpi, the NS group was injected with 0.3ml sterile normal saline. The mortality rate was evaluated and serum samples were taken for cultivation of the mycoplasma. The expression of Fas/FasL in mice thymus was analyzed with immunohistochemistry. The ul- trastructural changes of thymus, heart, lung, liver and kidney were observed with electron microscopy. Results The mice in ex- perimental group all died （24/24） within 3-5 days after infection. The expression of Fas / FasL in thymocytes was high, which was consistent with the extent of damage in the heart, liver, lung and kidney. Conclusion The high expression of Fas/FasL in thymo- cytes is correlated with the multi-organ damage in Mf and Mpi infected mice,

关 键 词：发酵支原体梨支原体胸腺多器官损伤Fas FasL 

分 类 号：R644[医药卫生—外科学]