珠眉海棠(Malus zumi Mats)盐应答MzSTO基因的克隆与表达分析  

Cloning and expression analysis of salt stress MzSTO gene from Malus zumi Mats

作　　者：蒋玉妆[1] 李凌子[1] 赵宇[1] 李擎天[1] 梁占锋[1] 孔瑾[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国农业大学学报》2013年第1期88-93,共6页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(31171940);北京市自然科学基金资助项目(6112012)

摘　　要：通过微列阵芯片(Microarray)分析,从珠眉海棠盐胁迫cDNA文库中分离得到MzSTO(Salt toleranceprotein)全长基因。结果表明:MzSTO基因cDNA全长1 066bp,开放阅读框共729bp,5′-UTR和3′-UTR的长度分别是49 bp和288 bp;MzSTO编码242个氨基酸,N端有2个保守的B-box锌指结构域(CX2CX8CX7CX2CX4HX8H);半定量RT-PCR表明MzSTO基因受到盐和干旱胁迫抑制,受冷处理诱导,并响应ABA(abscisic acid)。以上结果表明MzSTO基因可能通过依赖ABA的途径参与非生物逆境胁迫。The fuN-length cDNA sequence of MzSTO gene was isolated from a cDNA library of Malus zumi. The full- length cDNA of MzSTO was 1 066 bp. The 5＇-UTR and the 3＇-UTR were 49 and 288 bp respectively. Its open reading frame of 729 bp encoded a protein of 242 amino acids. There were two B-box zinc-finger domains （CX2CX8CX7CX2CX4HXSH） near N-terminal. MzSTO was induced by low temperature stress and repressed by high- salinLty and drought treatment, whLch was, also responsLve to ABA （abscisic acid） treatment by semi-quantative RT- PCR. All the results showed that MzSTO was involved in response of abiotic stresses including salt,drought and cold stresses through a possible ABA-dependent pathway in Malus zumi.

关 键 词：珠眉海棠 MzSTO 非生物胁迫 RT-PCR 

分 类 号：S661.4[农业科学—果树学]