峨眉山藏猕猴粪源大肠杆菌耐药性的调查研究  

Drug Resistance of Escherichia coli Strains Isolated from Macaca thibetana at Mt. Emei

作　　者：王钢[1] 伏秦超[1] 曹成全[1] 

机构地区：[1]乐山师范学院峨眉山生物多样性保护与利用研究所,四川乐山614004

出　　处：《四川动物》2013年第1期82-85,共4页Sichuan Journal of Zoology

摘　　要：目的了解峨眉山藏猕猴猴群大肠杆菌耐药性情况,为疫病防治和耐药性在野生猕猴群体中的扩散提供科学依据。方法采集峨眉山藏猕猴粪便105份,进行大肠杆菌的分离鉴定,并对分离杆菌进行致病性试验以及按照CLSI推荐的K-B药敏纸片法进行19种抗生素的耐药性检测。结果从105份样品中分离到105株大肠杆菌,其中6株致病性较强,23株有致病性,76株无致病性。各景区所分离的大肠杆菌对痢特灵(FUZ)耐药率较高,一线天达54.29%,钻天坡达39.47%,雷洞坪达46.87%。105株不同区域来源的大肠杆菌耐受的药物种数不同,一线天景区无12耐以上的菌株,而以3耐最多,为11.43%。钻天坡景区无7耐以上的菌株,而以1耐最多,为21.05%。雷洞坪景区无8耐以上的菌株,而以1耐最多,为18.75%。对各猴区分离的29株致病性大肠杆菌对19种抗生素共产生了7种耐药谱。总体来看,优势耐药谱不明显,且不同个体致病性大肠杆菌分离株的耐药谱存在差异。结论应定期追踪峨眉山藏猕猴大肠杆菌耐药情况,对野生藏猕猴疫病的防控具有比较重要的意义。Objective In order to provide scientific evidence for epidemic prevention and diffusion of drug resistance, analyzing of the drug resistance of Escherichia coli in Macaca thibetana at Mt. Emei was carried out. Method 105 macaque stool samples were collected for isolation and identification of E. coli applying pathogenic assay method. Antimicrobial susceptivity test were conducted by using the Kirby-Bauer method recommended by CLSI to examine the sensitivity against 19 antibiotics. Results The results indicated, 105 strains of E. coli were isolated including 6 strains of pathogenic stronger, 23 strains pathogenic, 76 strains of non pathogenic. E. coli isolated from different places showed different antibiotic resist- ance, and some strains displayed multiple drug resistance. In these E. coli strains, resistance to FUZ was common, and re- sistance level in scenic area was different, Yixiantian （54.29%） , Zuantianpo （39.47%）, Leidongping （46.87%）. The drug resistance of 105 strains is different in different scenic area. The E. coli showed no resistant to 12 antibiotics at Yixian- tian, and resistance to 3 antibiotics accounted for 11.43%. The E. coli showed no resistant to 7 antibiotics at Zuantianpo, and resistance to 1 antibiotics accounted for 21.05%. The E. coli showed no resistant to 8 antibiotics at Leidongping, and resistance to 1 antibiotics accounted for 18.75%. The 29 strains of pathogenic E. coli to 19 antibiotics provided 7 drug resistance pattens. Overall, advantages of resistance patterns are not significant, and there was different in different E. coli group. Conclusion Regular investigation of E. coli resistance to antibiotics was recommended, which was important to prevent and control of diseases for E. coli at Mt. Emei.

关 键 词：峨眉山藏猕猴 大肠杆菌 致病性 耐药性 

分 类 号：S858.9[农业科学—临床兽医学]