拟南芥MeIAA酯解酶基因表达模式的初步研究  

Study on expression pattern of Arabidopsis MeIAA esterase genes

作　　者：丁茂予[1,2] 侯仙慧[2] 刘赛男[2] 李林川[2] 陈章良[1,2] 康定明[1,2] 瞿礼嘉[2] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]北京大学生命科学学院/蛋白质和植物基因研究国家重点实验室,北京100871

出　　处：《中国农业大学学报》2013年第2期1-8,共8页Journal of China Agricultural University

基　　金：国家自然科学基金项目(31171564)

摘　　要：用植物生长素吲哚乙酸甲酯化酶酯解酶超家族部分成员基因启动子驱动GUS表达,研究了拟南芥MES超家族中3个有吲哚乙酸甲酯化酶酯解酶活性成员的基因表达模式。结果表明:1)AtMES16、AtMES17和AtMES18表达模式相似,新叶高,老叶低,从叶片中间向叶片边缘逐渐减少,类似吲哚乙酸甲基转移酶基因AtIAMT1的表达模式;2)AtMES17和IAMT1在叶片中同步但相互独立地表达,正常情况下AtMES17和IAMT1基因表达可能相互平衡制约。The expression patterns of three genes （AtMES16,AtMES17 and AtMES18） which possess MelAA esterase activity in the α-β hydrolase super-family of Arabidopsis genome were studied by using GUS report gene driven by promoters from the above three genes, respectively. The results showed that expression pattern of the three genes were similar with higher level in growing leaves, but lower in senescent leaves. On the other hand, the expression level was gradually attenuate from the middle vein to the edge of a leaf. Likewise, the AtlAMT1 showed the same expression pattern as that of the above three genes. Further observation indicated that the expression pattern of AtMES17 and AtlAMT1 were in an independent and synchronous way. Accordingly, it is conceivable that the expression of AtMES17 and IAMT1 should be interacted each other to keep a homeostasis regulation.

关 键 词：拟南芥 吲哚乙酸 酯解酶 表达模式 

分 类 号：Q786[生物学—分子生物学]