中国梨木虱冬型成虫性信息素成分的提取和GC-MS鉴定  

Extraction and GC-MS identification of sex pheromone components from winterform adults Cacopsylla chinensis(Yang et Li)

作　　者：万津瑜[1] 周玲[1] 张青文[1] 徐环李[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国农业大学学报》2013年第2期79-85,共7页Journal of China Agricultural University

基　　金：国家现代农业(梨)产业技术体系资助项目(CARS-29-08)

摘　　要：通过鉴定中国梨木虱性信息素的成分,为进一步开发其性引诱剂提供基础资料。本试验利用正己烷分别提取梨木虱冬型雌、雄成虫体表浸提物,用Y型嗅觉仪测试了浸提物对中国梨木虱冬型雌和雄成虫行为反应的影响,并运用气相色谱-质谱联用仪对浸提物进行了分析和鉴定。雌虫浸提物对雄性梨木虱有较强的引诱作用,雄虫浸提物对雄木虱,雌、雄浸提物对雌木虱均无引诱作用,说明雌虫浸提物中含有雌木虱释放的性信息素。气相色谱-质谱联用仪(GC-MS)分析结果表明,雌虫浸提物中13-甲基二十七烷和2-甲基二十七烷的含量多于雄虫浸提物中的含量。在保留时间为23.136min时,雌虫浸提物出现色谱峰,而雄虫浸提物没有出峰。这3种化合物可能是中国梨木虱冬型雌成虫的性信息素成分。The sex pheromone components from C. chinensis were identified to provide a basis for further development of sex attractant. In this paper, cuticular extracts were prepared by using hexane as solvent from winterform C. chinensis male and female adults, respectively. The effects of the extracts on the selective response of winterform males and females were tested in detail with a Y-tube olfactometer. The components of the extracts were analyzed and identified by GC-MS. The results showed that female extracts were attractive to male adults of C. chinensis （df= 1, P = 0. 016）, but male extracts were not. The male and female extracts didn＇t attract females as well, which indicated that female extracts could contain volatile sex pheromone released by female adults. Based on GC-MS data of authentic standards,the contents of 13-methylheptacosane and 2-methylheptacosane in female extracts were more than that in male extracts. There was a peak of the female extracts at the retention time of 23. 136 min, but none peak on that of male. The cureent results suggested that these compounds might be the sex pheromone active components from the winterform female adults of C. chinensis.

关 键 词：中国梨木虱 性信息素 Y型嗅觉仪 行为反应 气相色谱-质谱联用仪 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]