无溶剂体系中酶促合成中碳链甘三酯  

Enzymatic synthesis of medium chain triglycerides in solvent-free system

作　　者：马传国[1,2] 仝莹莹[1] 王向坡[1] 王业涛 柴小超[1] 

机构地区：[1]河南工业大学粮油食品学院,郑州450001 [2]小麦和玉米深加工国家工程实验室,郑州450001 [3]山东华建建设有限公司,山东聊城252000

出　　处：《中国油脂》2013年第2期48-51,共4页China Oils and Fats

摘　　要：在无溶剂体系下,采用Lipozyme 435催化甘油和中碳链脂肪酸(辛酸和癸酸混合物)酯化合成中碳链甘三酯(MCT)。结果表明在无溶剂体系下,反应温度对MCT得率具有显著影响。通过单因素试验和正交试验,得出制备MCT的最佳工艺条件为:反应温度90℃,加酶量(以脂肪酸质量计)5%,底物摩尔比(脂肪酸与甘油摩尔比)3∶1,反应时间16 h。在此条件下,MCT得率为92.10%,产物中甘二酯、甘一酯和游离脂肪酸含量分别为6.03%、0.13%和1.74%。The medium chain triglycerides （MCT） was synthesized by medium chain fatty acids （mixture of caprylic acid and capric acid） and glycerol with Lipozyme 435 as catalyst in solvent -free system. The results showed that the reaction temperature strongly affected MCT yield in solvent -free system. The op- timal process conditions of producing MCT were obtained by single factor experiment and orthogonal ex- periment as follows ： reaction temperature 90 ~C, dosage of enzyme 5% （based on the mass of fatty acid）, substrate molar ratio （molar ratio of fatty acid to glycerol ） 3： 1, reaction time 16 h. Under the optimal conditions, the MCT yield was 92. 10%, and the contents of diglycerides, monoglycerides and free fatty acids in the product were 6. 03%, 0. 13% and 1.74%, respectively.

关 键 词：LIPOZYME 435 中碳链甘三酯 酯化 

分 类 号：TQ641[化学工程—精细化工] TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]