四种α-取代丙酸酯在环糊精气相色谱固定相上的手性拆分机理  

Enantioseparation mechanism of several propanoic acid esters with α-substitute in capillary gas chromatography using cyclodextrin stationary phase

作　　者：申刚义[1] 张爱芹[2] 杨新玲[3] 凌云[3] 

机构地区：[1]中央民族大学中国少数民族传统医学国家民委-教育部重点实验室,北京100081 [2]中央民族大学生命与环境科学学院,北京100081 [3]中国农业大学应用化学系,北京100094

出　　处：《分析试验室》2013年第3期1-4,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(81001595)资助

摘　　要：计算了4种α-取代丙酸酯衍生物在β-环糊精衍生物气相色谱固定相上手性分离过程中的热力学参数,对手性拆分机理进行了探讨。实验结果显示随着温度的升高,样品的容量因子、手性选择因子和分离度都在逐渐降低。热力学参数表明,4种α-取代丙酸酯在固定相上的色谱保留及手性识别都为焓驱动过程,氢键力没有在手性识别中起到决定作用,而样品的母体结构和取代基团的性质则对手性识别有较大的影响。样品在固定相上的手性分离效率的驱动力主要来自于其对映异构体与固定相之间作用力的能量差异。The enantioseparation mechanism of four propanoic acid esters with α-substitute, in capillary gas chromatography using β-cyclodextrin derivative stationary phase was evaluated based on thermodynamic method. It was found that the capacity factor, chiral selectivity factor and resolution of samples were all decreased while the separation temperature increased. The results indicated the hydrogen bonding action was not the key to the enantioseparation. By comparison, the host structures and character of samples had larger effect on enantioseparation. The thermodynamic mechanism indicated further that enantioseparation was enthalpy controlling process in the examined temperature range mainly. Moreover, the efficiency of enantioseparation was maybe due to the difference of interaction energy between the enantiomers and the stationary phase.

关 键 词：毛细管气相色谱 Β-环糊精衍生物 α-取代丙酸酯 热力学 拆分机理 

分 类 号：O657.7[理学—分析化学]