罗丹明型简易Pd^(2+)高选择性增强型荧光探针  

A facile and highly selective fluorescence-enhanced Pd^(2+) probe derived from rhodamine fluorophore

作　　者：袁泽利[1] 乐清华[1] 江波[1] 吴庆[1] 胡庆红[1] 张铭钦[1] 张成江[1] 

机构地区：[1]遵义医学院药学院,遵义563003

出　　处：《分析试验室》2013年第3期38-42,共5页Chinese Journal of Analysis Laboratory

基　　金：贵州省国际科技合作项目(黔科合外G字[2012]7036);贵州省科学技术基金项目(黔科合J字[2011]2032);教育部春晖计划项目(教外司留[2012]600)资助

摘　　要：用罗丹明B与水合肼一步反应简便制得罗丹明衍生物RBH,试验发现:在乙腈-水(CH3CN:H2O=4:1)溶液中,RBH对Pd2+具有高选择性和广泛的pH探测范围,其对常见金属离子不敏感,且常见钠、钾盐阴离子对Pd2+探测也无干扰作用。探针RBH与Pd2+结合后,RBH的吸收和荧光强度显著增加,其荧光强度增加24倍,检测时间仅需25 min。该Pd2+探针响应灵敏,最低检出限为1.76×10-6mol/L。RBH对Pd2+的识别过程为不可逆,且该探针可制成试纸检测mmol/L级别含Pd2+水样。RBH has been synthesized by a facile one step coupling of rhodamine B with hydrazine hydrate. We incidentally found that RBH showed high selectivity toward Pd^2＋ in acetonitrile-water solutions （CH3 CN：H2O = 4：1 ） with a broad pH span （5.58 -12.23 ） but no significant response toward other competitive cations, such as Fe^2＋ , Co^2＋ , Ni^2＋ , Cu^2＋ , Zn^2＋ , Pb^2＋ , Cd^2＋ , Ca^2＋ , Mg^2＋ , K^＋ , Na^＋ , etc. No interference was also observed with common anions like Ac^ - , Br^- , C1^ - , ClO4^ - , F ^- , H2PO4^ - , I^ - , HSO4^ - , NO3^-and SCN - （ as their Na^＋ or K^＋ salts）, respectively. RBH also showed responses to Pd^2＋ within 25 minutes. The fluorescent intensity was enhanced 24 times. Additionally, RBH has the detection limit of 1.76 × 10^-6mol/L suggesting its high sensitivity for Pd^2＋ detection. Moreover, the recognit ion of Pd^2＋ was not reversible, and RBH can also be loaded as test paper for detectiong Pd^2＋ with mmol/L level in water solution.

关 键 词：罗丹明水合肼 荧光探针 钯离子 荧光增强 

分 类 号：O657.3[理学—分析化学]