荧光探针法检测人体代谢中头孢他啶含量  

Determination of ceftazidime content in the metabolism of human body by fluorescent probe

作　　者：程定玺[1] 杨璐[1] 梁宇[2] 

机构地区：[1]河南师范大学化学化工学院,新乡453007 [2]新乡学院化学与化工学院,新乡453007

出　　处：《分析试验室》2013年第3期43-46,共4页Chinese Journal of Analysis Laboratory

基　　金：河南省重点科技攻关项目(102102210043)资助

摘　　要：建立了一种快速测定头孢他啶含量的荧光探针法。在pH 4.00的Britton-Robinson缓冲溶液中,曙红Y与十六烷基三甲基溴化铵发生荧光增强反应,再加入头孢他啶,体系荧光强度降低,且降低程度与药品加入量有良好线性关系。在优化实验条件下,线性范围为0.008～0.8 mg/L,检出限为0.0018 mg/L。借此检测了头孢他啶在人体代谢(血清和尿液)中的含量,回收率97.5%～102.6%,相对标准偏差1.9%～2.1%。A new simple and fast method for the determination of ceftazidime （CAZ） was developed. In the Britton-Robinson（B-R） buffer solution at pH 4. 00, the fluorescence intensity of 2,4,5 -7-tetrabromofluorescein disodium salt （EY） is enhanced with addition of certain concentration of the cetyl trimethyl ammonium bromide （CTMAB）. The enhanced fluorescence intensity declined obviously after adding ceftazidime and the reduced fluorescence intensity was proportional to the concentration of ceftazidime. Under optimal experimental conditions, the linear range is 0. 008-0. 8 mg./L, and the detection limit is 0. 0018 mg/L. The system detected the content of ceftazidime in the metabolism of human body （ serum and urine）, was detected by this method, and the average recoveries of the method ranged from 97.5% - 102.6% with the relative standard deviation （RSD） range of 1.9% - 2. 1%.

关 键 词：荧光 曙红Y 十六烷基三甲基溴化铵 头孢他啶含量 

分 类 号：O657.3[理学—分析化学]