毛细管电泳非接触电导法测定4种中枢神经系统用药  

Determination of four central nervous system drugs by capillary electrophoresis with contactless conductivity detection

作　　者：朱妍[1] 葛淑丽[1] 汪雪[1] 舒露[1] 王清江[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200241

出　　处：《分析试验室》2013年第3期73-76,共4页Chinese Journal of Analysis Laboratory

基　　金：科技部科学仪器设备重大专项(2011YQ150072)资助

摘　　要：运用毛细管电泳非接触式电导检测方法对4种中枢神经系统用药-盐酸阿扑吗啡、氢溴酸加兰他敏、富马酸喹硫平、氯氮平的分离进行了研究。考察了电泳介质的种类、浓度、分离电压、进样时间对分离效果的影响,在10 mmol/L三羟基氨基甲烷(Tris)-8 mmol/L柠檬酸(Cit)-20%甲醇的运行缓冲液中,激发电压为60V,激发频率为600kHz,4种药物在15 min内得到了分离。4种药物的线性范围分别为0.97～15.6 mg/L;0.97～15.6 mg/L;0.48～15.6 mg/L和0.97～250 mg/L,检测限为0.32,0.32,0.16和0.32 mg/L。A new method has been built for simultaneous determination of apomorphine hydrochloride, galanthamine hydrobromide, quetiapine fumarate and clozapine in tablet by capillary electrophoresis with contactless conductivity detection. The effects of some important factors such as the concentration of running buffer,separation voltage, injection time and additives were investigated. With optimized buffer of 10 mmol/L Tris-8 mmol/L Cit-20% methanol, the samples were well separated and detected at the excitation voltage of 60V and frequency of 600kHz. The linear ranges of apomorphine, galanthamine, quetiapine and clozapine were 0. 97 - 15.6 mg/L; 0. 97 - 15.6 mg/L; 0. 48 - 15.6 mg/L and 0.97-250 mg/L, respectively. The detection limit were 0.32, 0. 32,0. 16 and 0.32 mg/L.

关 键 词：毛细管电泳 非接触式电导 盐酸阿扑吗啡 氢溴酸加兰他敏 富马酸喹硫平 氯氮平 

分 类 号：O657.8[理学—分析化学]