α-细辛醚对癫痫大鼠的海马钙稳态调控的影响  

Effects of α-asarone on modulation of hippocampus calcium homeostasis in epilepsy rats

作　　者：苗静琨[1] 陈启雄[2] 李春[2] 李晓文[2] 刘振球[2] 吴小玫[3] 张晓萍[4] 

机构地区：[1]重庆医科大学附属儿童医院儿童发育疾病研究省部共建教育部重点实验室,重庆400014 [2]重庆医科大学附属儿童医院新生儿科,重庆400014 [3]重庆医科大学附属儿童医院儿科学重庆市重点实验室,重庆400014 [4]重庆医科大学附属儿童医院重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014

出　　处：《中国临床药理学杂志》2013年第2期139-141,共3页The Chinese Journal of Clinical Pharmacology

基　　金：重庆医科大学博士启动基金资助项目(0200101060)

摘　　要：目的研究α-细辛醚对癫痫大鼠的海马钙稳态的影响。方法大鼠癫痫持续状态(SE)后,观察α-细辛醚对海马游离Ca2+浓度、游离钙调蛋白(CaM)平均通道荧光值、总CaM及钙调蛋白依赖性蛋白激酶(CaMK)IIa-mRNA表达的变化的影响。结果模型组SE后,各时相海马游离Ca2+显著增高(P<0.05);CaM平均通道荧光值显著降低(P<0.05);SE后12～72 h,游离CaM表达较正常组明显增强(P<0.05);CaMK IIa-mRNA表达显著降低(P<0.05)。与模型组相比,α-细辛醚组游离Ca2+浓度显著降低,游离CaM平均通道荧光值明显增加,总CaM表达显著降低,CaMK IIa-mRNA表达明显增强(P<0.05)。结论α-细辛醚可下调SE后海马游离Ca2+浓度及结合CaM表达,并上调CaMK IIa-mRNA表达。Objective To observe the effects of α - asarone on hippo- campus calcium homeostasis in in epilepsy rats. Methods Rat status epilepticus （SE） model was induced by Lithium -Pilocarpine. The effects of α - asarone on the content of intracellular free Ca2＋ , the average passage fluorescence of relative activity of calmodulin （CaM） and the expression of the CaM and Ca2＋/CaM -dependent kinase IIa （CamK IIa） in hippocampus of post -SE rats were observed. Results The intracellular free Ca2＋ content was significantly increased in the hippoeampus of epilepsy rats （P 〈 0. 05）. The mean channel fluorescence of intra- cellular free CaM was significantly decreased at different time post -SE （P 〈 0. 05 ）. At 12 - 72 h post - SE, the CaM expression in model group was significantly higher than those in normal group, and still higher than in normal group at one week post - SE. The expression of CaMK IIa - mRNA was significantly decreased at different time in the hippoeampus of post - SE rats （ P 〈 0. 05 ） , and still lower than in normal group at one week post - SE. The content of intracellular free Ca2 ＋ and the expression of CaM in α - asarone group were significantly lower than those in model group at different time in the hippoeampus of post - SE rats （P 〈 0.05）. The mean channel fluorescence of intraeellular free CaM and the expression of CaMK IIa - mRNA in α - asarone group were significantly higher than those in model group （P 〈 0. 05 ） . Conclusion α - asarone could significantly downregulated the intracellular free Ca2＋ content and the ex- pression of CaM and upregulated the expression of CamK IIa in the hippocampus of post - SE.

关 键 词：癫痫 Α-细辛醚 钙调蛋白 

分 类 号：R971[医药卫生—药品] R742.6[医药卫生—药学]