LC-MS/MS法测定人血浆中的凡德他尼  

Determination of vandetanib in Chinese cancer patients by LC-MS/MS

作　　者：詹靖[1,2] 李苏[1,2] 廖海[1,2] 邓丽婷[1,2] 

机构地区：[1]华南肿瘤学国家重点实验室,广州510060 [2]中山大学肿瘤防治中心,广州510060

出　　处：《中国临床药理学杂志》2013年第2期145-147,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的建立LC-MS/MS测定人血浆中凡德他尼浓度的方法。方法用甲基-叔丁醚液液萃取法处理血浆样品,色谱柱:Intersil ODS-3 RP18柱;流动相:0.02 mol.L-1乙酸铵缓冲液(pH=3)-乙腈(70∶30);流速:200 mL.min-1;电喷雾电离源(ESI)离子化,多反应离子监测方式测定。结果凡德他尼血药浓度在5～1000 ng.mL-1范围线性良好,低、中、高浓度的绝对回收率分别为95.4%,102.4%,97.2%;批内、批间精密度良好(RSD<10%)。结论该方法稳定、准确、灵敏、快速、特异性强,可用于该药临床药代动力学研究。Objective To establish a HPLC -MS/MS method in order to determine vandetanib in human plasma. Methods The Intersil ODS -3 RP18 column was used as analytical column with a mobile phase consisted a mixture of ammonium acetate （ 0. 02 mol · L- 1 , pH = 3 ） and acetonitrile （70： 30） , the flow rate was 200 mL · min-1. The plasma sample was wiped off protein with methyl tert - butyl ether （ MTBE ）. Quantification was performed with positive ion MS/MS employing a TurboIonSpray interface. Results The linear range was 5 - 1000 ng · mL-l ~ The absolute recovery of 15, 500, 800 ng mL-1 was 95.4%, 102.4% ,97.2% , respectively. The intra -batch and inter -batch deviations were in high precision （ RSD 〈 10% ）. Conclusion The method is proved to be stable, sensitive, accurate, rapid, specific and suitable for the clinical pharmacokinetics study.

关 键 词：LC-MS MS 凡德他尼 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R979.1[医药卫生—药学]